Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?

Yes. Cells can be frozen after the wash step with Buffer FCW in Appendix D Protocol 'Processing Suspended Cells' of the FastLane Cell cDNA Handbook. Buffer FCW should be aspirated promptly after pelleting the cells during the wash step. Following the removal of Buffer FCW at step D5, the cells can be frozen. For future processing, thaw the cell pellet, and continue with the lysis step D6 using Buffer FCP.


Can’t find what you are looking for?

Browse the FAQ base with our FAQ search.