Cat. No. / ID: P7610L
VeraSeq PCR Mix is a premixed, ready-to-use 2x solution containing VeraSeq 2.0 High-Fidelity DNA Polymerase, dNTPS, MgCl2 and reaction buffer at optimal concentrations to maximize the speed, accuracy and length of DNA synthesis. The formulation provides efficient, high-fidelity DNA amplification, cloning, and synthetic biology applications.
|Functional Assay||Amplification of 500 bp fragment from Genomic DNA|
Source of recombinant enzyme protein
The protein is produced by a recombinant E. coli strain carrying the engineered VeraSeq 2.0 gene.
One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble form at 74°C in 30 minutes.
General precautions should be taken when setting up a PCR, including setting up the reaction on ice, adding master mix last, gently pipetting, thorough mixing and a quick centrifugation. The following procedure can be used as a guideline. Reactions may need to be optimized individually.
Reaction setup (for 50µl)
|Component||Volume (µl)||Final concentration|
|Sterile H2O||20, variable||n/a|
|2x VeraSeq PCR Mix||25||1x|
|PCR Primer Cocktail||5||0.5 µM each|
* Total reaction volumes of library DNA and water should be adjusted to achieve a final reaction lolume of 50µl. If the reaction volume needs to be >50 µl, the volume of the 2x Master Mix should be adjusted so that it constitutes 50% of the final reaction volume.
Typical cycling conditions*
|Initial denaturation||98°C||30 seconds||1|
|To be determined by user†|
* Cycling conditions may need to be optimized, depending on the amplicon of interest.
† Number of cycles is dependent on the amount of input DNA and other specific sequence analysis requirements.
Quality control analysis
Functionality of 2x VeraSeq PCR Mix is assessed by its ability to amplify a 500 bp fragment from genomic DNA. Following PCR the 500 bp fragment was visualized by agarose-gel electrophoresis.
VeraSeq PCR Mix was tested prior to assembly and found to be free of contaminating endonucleases. Enzyme purity was >99% as determined by SDS-PAGE and negligible E. coli genomic DNA contamination was confirmed by qPCR. Specific activity was verified pre and post dilution.
This OEM by QIAGEN product is available for bulk purchase for the following commercial assay applications.