Cat. No. / ID: 9001600
The Rotor-Disc 72 Starter Kit includes all you need for runs of 72 reactions on the Rotor-Gene Q real-time cycler.
The Rotor-Disc 72 Starter Kit enables easy start up of real-time PCR experiments on the Rotor-Gene Q using the Rotor-Disc 72 format. The kit includes Rotor-Disc 72 packs, a Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, a Rotor-Disc 72 Rotor and Locking Ring, a Rotor-Disc 72 Loading Block, and a Rotor-Disc Pipetting Aid.
Rotor-Discs are fully compatible with the QIAgility instrument, which enables automated reaction setup.
The Rotor-Disc 72 Rotor and Rotor-Disc 72 Locking Ring are used with the Rotor-Disc 72. The Rotor-Disc 72 is a circular plate of 72 vertically oriented reaction wells for high-throughput use.
The Rotor-Disc 72 does not use caps. Instead, Rotor-Disc Heat Sealing Film is applied to the top and heat sealed using a Rotor-Disc Heat Sealer. The film prevents contamination by providing a strong, durable, and tamper-proof seal.
The Rotor-Gene Q and Rotor-Disc 72 Starter Kit enable real-time PCR and high-resolution melting (HRM) applications, including:
No. The master mixes in Rotor-Gene Kits contain dTTP instead of dUTP. If UNG treatment is required, we recommend using QuantiTect +UNG Kits. QuantiTect Kits are also compatible with the Rotor-Gene Q; however, the kits require a significantly longer cycling time.
The Rotor-Gene Q instrument can be used with two different rotor formats: using tubes or rotor-dics. Tubes can be run in 36 and 72-well rotors and rotor-discs in Rotor-Disc 72 and Rotor-Disc 100 rotors. When programming the temperature profile please make sure the correct rotor type is selected.
The majority of the Rotor-Gene Kit data shown in our literature has been generated with the help of the QIAgility instrument. We did not observe any problems during the pipetting steps.
Rotor-Gene Kits are specifically developed for the Rotor-Gene Q PCR Cycler. The unique rotary system of the cycler combined with the kits’ proprietary buffer system enable ultrafast cycling. Rotor-Gene Kits do not contain ROX dye since no normalization to a passive reference is required. Also, Rotor-Gene Kits do not contain dUTP; therefore, UNG pretreatment is not possible.