QIAshredder

For simple and rapid homogenization of cell and tissue lysates

Features

  • Replaces syringe-and-needle homogenization
  • Reduces loss of sample material
  • Eliminates cross-contamination between samples
  • Filters out insoluble debris and reduces viscosity
QIAshredder (250)

Cat. No. / ID: 79656

250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps
$418.00
Pieces
250
50
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The QIAshredder is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The QIAshredder homogenizer consists of a unique biopolymer-shredding system in a microcentrifuge spin-column format. Cell or tissue lysate is loaded onto the QIAshredder homogenizer placed in a collection tube and centrifuged. The homogenized lysate is then collected. In general, similar yields and quality of RNA are obtained as with rotor–stator homogenization (see figure " Comparison of RNA yields").
See figures

Supporting data and figures

Resources

Kit Handbooks (1)
RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi
Gene Expression Analysis (1)

Publications

The protective role of a small GTPase RhoE against UVB-induced DNA damage in keratinocytes.
Boswell SA; Ongusaha PP; Nghiem P; Lee SW;
J Biol Chem; 2006; 282 (7):4850-4858 2006 Dec 14 PMID:17170105
Cell specific patterns of methylation in the human placenta.
Grigoriu A; Ferreira JC; Choufani S; Baczyk D; Kingdom J; Weksberg R;
Epigenetics; 2011; 6 (3):368-79 2011 Mar 1 PMID:21131778
Mechanisms of confluence-dependent expression of CD26 in colon cancer cell lines.
Abe M; Havre PA; Urasaki Y; Ohnuma K; Morimoto C; Dang LH; Dang NH;
BMC Cancer; 2011; 11 :51 2011 Feb 1 PMID:21284881
Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-κB ligand expression in MG-63 osteoblast-like cells exposed to polyethylene particles.
Xu J; Kauther MD; Hartl J; Wedemeyer C; Study was performed at the University of Duisburg - Essen, Germany.;
J Orthop Surg Res; 2010; 5 :83 2010 Nov 4 PMID:21050480

FAQ

What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?

Yield and integrity of genomic DNA depend on the disruption and homogenization method used with the Allprep DNA/RNA Kits.

Homogenization with the TissueRuptor (or other rotor–stator homogenizer, such as the Polytron) or the TissueLyser results in greater DNA fragmentation (depending on homogenization time and intensity). However, shorter DNA fragments are easier to elute, so DNA yields will be higher.

In contast, homogenization using the QIAshredder (or a syringe and needle) is more gentle, resulting in less DNA fragmentation. However, longer DNA fragments are more difficult to elute, so DNA yields may be lower.

 

FAQ ID -1751
What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?

The QIAshredder is a unique biopolymer shredding system in a microcentrifuge spin-column format. It homogenizes cell or tissue lysates to reduce viscosity. Homogenization shears the high-molecular weight genomic DNA and other high-molecular-weight cellular components to create a homogenous lysate. The QIAshredder is chemically inert and will not bind nucleic acids.

It cannot replace tissue disruption or enzymatic cell wall lysis by mechanical and chemical methods, respectively. Both, efficient cell wall disruption and lysate homogenization are fundamental for successful RNA isolation from all types of samples. Once complete disruption has been achieved, the QIAshredder Homogenizer can be used in place of needle and syringe, or rotor-stator homogenization.

For further information regarding sample disruption and homogenization please refer to the 'Disruption and homogenization of starting materials' section in the RNeasy Mini Handbook, and see FAQ 139.

 

FAQ ID -631
Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?

The QIAshredder Maxi spin columns  from the DNeasy Plant Maxi Kit are not recommended for use in conjunction with QIAGEN's RNA isolation products. The filter can only tolerate low centrifugal forces not sufficient to shear genomic DNA. If genomic DNA is not sheared, there will be an increase in genomic DNA contamination in the purified RNA.

For an alternative to QIAshredder homogenization in combination with RNeasy Midi/Maxi Preps, please see FAQ 560.

FAQ ID -616
Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?
We only offer QIAshredder spin columns for use with the RNeasy Mini Kit. When working with RNeasy Midi/Maxi, we recommend using a rotor-stator, or mortar and pestle with needle and syringe homogenization.
FAQ ID -560
Can I homogenize samples in QIAzol using the QIAshredder?
Yes
FAQ ID -501