Investigator Argus X-12 QS Kit

For multiplex amplification of 12 X-chromosomal STRs, D21S11 and Amelogenin, with an innovative Quality Sensor

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Investigator Argus X-12 QS Kit (100)

Cat. No. / ID: 383225

Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, allelic ladder Argus X-12 QS, DNA size standard 550 (BTO) and Nuclease-Free Water
$2,396.00
Reactions
100
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The Investigator Argus X-12 QS Kit is intended for molecular biology applications in forensic, human identity and paternity testing. This product is not intended for diagnosis, prevention or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Co-amplification of 12 X-chromosomal and one autosomal alignment marker to avoid sample mix-up
  • Integrated Quality Sensor for better decision making and data interpretation
  • PCR run speed of around 80 minutes for faster results
  • Smart primer design with reduced allelic overlap for unambiguous results
  • High sensitivity and inhibitor resistance

Product Details

The Investigator Argus X-12 QS Kit is a multiplex solution for human identification. The 12 X-chromosomal markers, D21S11 and Amelogenin are co-amplified. The autosomal marker, D21S11, enables alignment of the Investigator Argus X-12 QS Kit profile with a profile from any other autosomal STR kit from the expanded CODIS or ESS market, to minimize the risk of sample mix-ups. With its innovative Quality Sensor (QS), the Investigator Argus X-12 QS Kit includes a novel internal performance control. This allows you to readily determine if your PCR run was successful or if there was no DNA present in your sample. The kit utilizes QIAGEN's fast-cycling technology, enabling amplification in just ~80 minutes. Highly sensitive, with rapid and reliable results from trace or reference DNA, the Investigator Argus X-12 QS Kit is suitable for paternity or kinship testing and for gonosomal STR analysis of forensic stains.

Performance

The Investigator Argus X-12 QS Kit utilizes fast-cycling technology to provide reliable results in approximately 80 minutes.
The novel Fast Reaction Mix 2.0 is based on proven QIAGEN Multiplex PCR technology that has been further optimized for direct amplification. Assay performance tests in the presence of increasing concentrations of inhibitors showed superior inhibitor resistance. This means reliable, high-quality results.
The Investigator Argus X-12 QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.

Principle

The Investigator Argus X-12 QS Kit uses QIAGEN's fast-cycling PCR technology for the direct, simultaneous amplification of 12 X-chromosomal markers, D21S11 and Amelogenin. The amplification of the 12 X-chromosomal markers (see Table 1 and 2), with heightened discrimination power, meets the needs for complex kinship and paternity testing and helps fulfill the demands of complicated deficiency cases involving at least one female. The 12 X-chromosomal markers are clustered into four linkage groups (three markers per group; Table 1), and thus each set of three markers is handled as a haplotype for genotyping.

Table 1. Linkage groups
Linkage group
1 (Xp22) DXS8378 – DS10135 – DXS10148
2 (Xp11) DXS7132 – DXS10074 – DXS10079
3 (Xp26) HPRTB – DXS10101 – DXS10103
4 (Xp28) DXS7423 – DXS10134 – DXS10146


The innovative Quality Sensors provides additional information on the sample quality and PCR success beyond the profile of your sample. With the Quality Sensor (QS) being amplified as an amplification control, it is possible to determine:

  • A successful PCR run
  • A failed PCR run
  • Lack of DNA in your sample


This additional information will help you analyze your data, and guides you with respect to further sample processing. It will also help you reduce unnecessary PCR re-runs, saving you time and money.
The additional autosomal marker D12S11 acts as alignment marker. Now, you have the possibility to align your X-chromosomal profile from the Investigator Argus X-12 QS Kit with a profile of any other autosomal STR kit from the expanded CODIS or ESS market, to minimize the risk of sample mix-ups.

Procedure

The 12 X-chromosomal markers, D21S11 and the gender-specific Amelogenin, are amplified simultaneously in a single PCR run and labeled with one of the dyes listed in the Table 2: "Investigator Argus X-12 QS Kit dyes and corresponding markers".
The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the table "Technical specifications" for more information.

Table 2. Investigator Argus X-12 QS Kit dyes and corresponding markers
Dyes Markers
6-FAM QS1 Amelogenin DXS10103 DXS8378 DXS10101 DXS10134
BTG DXS10074 DXS7132 DXS10135      
BTY DXS7423 DXS10146 DXS10079      
BTR DXSHPRTB DXS10148 D21S11      

Table 3. Investigator Argus X-12 QS Kit technical specifications
Category Details
Volume per PCR 25 µl
Matrix BT5
Fluorescence labels 6-FAM, BTG, BTY, BTR, BTO
Genetic analyzers ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl or
Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis Applied Biosystems GeneMapper ID, GeneMapper ID-X or Genotyper Software

Applications

The Investigator Argus X-12 QS Kit enables simultaneous amplification of 12 X-chromosomal markers, and is highly suitable for:
  • Kinship and paternity testing, especially deficiency cases
  • Population genetics and anthropological studies
  • Analysis of stains with female traces on a male background

Resources

Brochures & Guides (2)
Advanced sample collection, automation and STR solutions for kinship testing
Technical Information (5)

The most comprehensive and versatile coverage of current international STR standards


Enjoy success at first try with high performance QIAGEN Multiplex STR amplification and instrumentation

Quality initiatives for human identity testing and forensics 
Application/Protocol Documents (1)
For the preparation of SeqStudio Genetic Analyzer to perform electrophoresis using Investigator STR Assays

July 2022
Template Files (3)
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Quick-Start Protocols (1)
Kit Handbooks (1)