QIAcard FTA Elute Buffer

For improved recovery and integrity of nucleic acids from QIAcard FTA Elute format

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QIAcard FTA Elute Buffer

Cat. No. / ID: WB120100

For 200 preps of, e.g., 4 x 3 mm (Ø) punches of QIAcard FTA Elute formats.
$186.00
QIAshredder/QIAGEN Proteinase K not included
Please note that QIAshredder and QIAGEN proteinase K are not included with the buffer.
The QIAcard FTA Elute Buffer is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Long term sample storage and improved elution of nucleic acids
  • Tailored protocols for different sample materials
  • Flexible sample scaling for various punch sizes and numbers
  • Reduction of degradation and higher elution efficiency

Product Details

The QIAcard FTA Elute Buffer is designed for improved elution of nucleic acids from QIAcard FTA Elute formats. DNA eluted using the QIAcard FTA Elute Buffer is immediately suitable for sequencing and PCR applications such as qPCR and RT-qPCR as well as subsequent STR analysis (standard amplification).

Easy sample collection of cellular material from saliva, buccal cells, blood and other biological material is important for downstream DNA analysis. Sample collection products must facilitate safe and easy transport of this material to the lab and deliver reliable long-term storage of samples for repeat testing. All the FTA paper-based products in the QIAGEN portfolio have longer storage data than any other similar product. Find out more here about sample collection with QIAcard FTA products and accessories.

Want to try the QIAcard FTA Elute Buffer for the first time? Register to start your trial.*

* The trial may not be available in your region.

Performance

FTA (Flinders Technology Associates) is a chemically coated cellulose matrix, capable of lysing cells upon contact and denaturing proteins. Sample materials include (but are not limited to) blood, buccal cells, tissue, cultured cells, microorganisms and plant tissue.

The QIAcard FTA Elute Buffer allows for the rapid elution of nucleic acids from QIAcard FTA Elute formats. Stored purified DNA bound to the matrix is eluted in 30 minutes. Nucleic acids from blood or saliva samples can be eluted in less than 40 minutes. The QIAcard FTA Elute Buffer simplifies the elution protocol and protects the nucleic acids from degradation. Moreover, the buffer is designed for combined treatment with Proteinase K and QIAshredder (not included; for ordering information, see cat. no. 19131 and cat. no. 79656). Use of the QIAshredder basket is recommended to improve the elution efficiency. Proteinase K treatment improves the efficiency of lysis and elution for challenging sample material such as biological fluids (blood, saliva, etc.).

Principle

Applied sample material (such as blood, buccal cells, tissue, cultured cells, microorganisms or plant tissue) is lyzed upon contact with QIAcard FTA Elute formats. QIAcard FTA Elute formats are suitable for room temperature (15–25°C) shipment and preservation. The cards are designed for subsequent elution of released and nucleic acids from the card matrix. The QIAcard FTA Elute Buffer simplifies the elution protocol, protects the nucleic acids from degradation and is designed for combined treatment with Proteinase K to improve the efficiency of lysis and elution.

Procedure

Sample material is applied to the printed circle area on an appropriate QIAcard FTA Elute format. When completely dried, the cards can be stored in a dry environment until ready to use. Punches from QIAcard FTA Elute formats are placed in a 2 ml microcentrifuge tube and washed with TE−4 buffer. The QIAcard FTA Elute Buffer procedure, with tailored protocols for purified nucleic acids or cells and biological fluids, is shown in the flowchart " QIAcard FTA Elute Buffer procedure".

See figures

Applications

The combination of QIAcard FTA Elute formats for easy sample collection, room temperature preservation and the use of QIAcard FTA Elute Buffer enable efficient nucleic acid elution for downstream applications such as:

  • Rapid DNA isolation
  • Biobanking
  • Genotyping real-time PCR, STR Analysis
  • Transgenics/genetic identification
  • Pharmacogenomics
  • Expression profiling using RT-qPCR (RNA)

Supporting data and figures

Resources

Kit Handbooks (1)
For elution of nucleic acids from QIAcard FTA Elute formats