QIAamp DSP DNA FFPE Tissue Kit

For purification of DNA from formalin-fixed paraffin-embedded tissues

Features

  • Rapid purification of DNA from formalin-fixed paraffin-embedded tissues
  • Consistent, high yields
  • Removal of contaminants and inhibitors
QIAamp DSP DNA FFPE Tissue Kit (50)

Cat. No. / ID: 60404

For 50 DNA preps: QIAamp MinElute columns, Proteinase K, Buffers, and Collection Tubes (2 ml)
$278.00
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The QIAamp DSP DNA FFPE Tissue Kit is intended for in vitro diagnostic use.

Product Details

The QIAamp DSP FFPE Tissue Kit provides silica-based DNA purification from formalin-fixed paraffin-embedded tissue. The QIAamp DSP DNA FFPE Tissue Kit is designed for labs that process FFPE tissues for in vitro diagnostic use.

Performance

The QIAamp DSP DNA FFPE Tissue Kit uses QIAamp MinElute spin columns for purification of high-quality DNA from FFPE tissue. Genomic DNA is efficiently purified from FFPE tissue sections without overnight incubation. The procedure partially removes formalin crosslinking of released DNA, for improved yield and DNA performance in downstream applications.

Principle

The QIAamp DSP DNA FFPE Tissue Kit uses well-established QIAamp MinElute technology for purification of genomic DNA from FFPE tissue. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 200 µl.

Specially optimized lysis conditions allow genomic DNA to be efficiently purified from FFPE tissue sections without the need for overnight incubation. Incubation at an elevated temperature after proteinase K digestion partially removes formalin crosslinking of the released DNA, improving yield as well as DNA performance in downstream assays. Note that DNA isolated from FFPE samples is usually of lower molecular weight than DNA from fresh or frozen samples. The degree of fragmentation depends on the type and age of the sample and the conditions used for fixation.

Procedure

The QIAamp DSP DNA FFPE Tissue Kit uses special lysis conditions to release DNA from tissue sections and to overcome inhibitory effects cause by formalin cross-linking of nucleic acids. The procedure consists of 6 steps — remove paraffin, lyse, heat, bind, wash, and elute (see "Procedure"). Paraffin is dissolved in xylene and removed. The sample is lysed under denaturing conditions, with a short proteinase K digestion. Incubation at 90°C reverses formalin cross-linking. DNA binds to the membrane and contaminants are washed away. DNA is eluted in Buffer ATE and is immediately ready for use in amplification reactions or for storage at –20°C. The simple QIAamp DSP DNA FFPE Tissue Kit procedure is highly suited for simultaneous processing of multiple samples.

Applications

The QIAamp DSP DNA FFPE Tissue Kit is specially designed for purifying DNA from formalin-fixed paraffin-embedded tissue.

Resources

Brochures & Guides (1)
High-quality, nucleic acid purification for successful PCR and NGS experiments.
Kit Handbooks (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728