QIAamp DSP DNA FFPE Tissue Kit
For purification of DNA from formalin-fixed paraffin-embedded tissues
- Rapid purification of DNA from formalin-fixed paraffin-embedded tissues
- Consistent, high yields
- Removal of contaminants and inhibitors
The QIAamp DSP FFPE Tissue Kit provides silica-based DNA purification from formalin-fixed paraffin-embedded tissue. The QIAamp DSP DNA FFPE Tissue Kit is designed for labs that process FFPE tissues for in vitro diagnostic use.
The QIAamp DSP DNA FFPE Tissue Kit uses QIAamp MinElute spin columns for purification of high-quality DNA from FFPE tissue. Genomic DNA is efficiently purified from FFPE tissue sections without overnight incubation. The procedure partially removes formalin crosslinking of released DNA, for improved yield and DNA performance in downstream applications.
The QIAamp DSP DNA FFPE Tissue Kit uses well-established QIAamp MinElute technology for purification of genomic DNA from FFPE tissue. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 200 µl.
Specially optimized lysis conditions allow genomic DNA to be efficiently purified from FFPE tissue sections without the need for overnight incubation. Incubation at an elevated temperature after proteinase K digestion partially removes formalin crosslinking of the released DNA, improving yield as well as DNA performance in downstream assays. Note that DNA isolated from FFPE samples is usually of lower molecular weight than DNA from fresh or frozen samples. The degree of fragmentation depends on the type and age of the sample and the conditions used for fixation.
The QIAamp DSP DNA FFPE Tissue Kit is specially designed for purifying DNA from formalin-fixed paraffin-embedded tissue.
It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.
Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.
Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.
Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.
Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).