Standardized viral vector processing and quantification using digital PCR
Quantitative PCR is a widely used method for AAV genome quantification, but it can lead to variability that is intolerable in cell and gene therapy (CGT). Instead, a standardized method for sample processing is needed to achieve accurate AAV quantification.
In this webinar, join Domenica Martorana, Senior Scientist at QIAGEN, as she introduces you to a dedicated workflow for capsid lysis and AAV titer quantification by digital PCR. Learn how a combination of the new CGT Viral Vector Lysis kits and CGT Digital PCR assays achieves:
- Easy vector genome titration
- Outstanding accuracy, reproducibility and speed
- A workflow comparable to qPCR or custom lab solutions
- Improved accessibility to targets of interest and increased genome titers over the whole vector genome
About the speaker
Dr. Domenica Martorana, R&D Senior Scientist
Dr. Domenica Martorana is an R&D senior scientist working on gene regulation product development at QIAGEN, Hilden, Germany. She studied Molecular and Cellular Biology at the Georg-August University in Göttingen (Germany), where she received her PhD in 2019. Her PhD research focused on the transcriptional regulation of the unfolded protein response in both fungi and higher eukaryotes. Along the way, she has gathered extensive experience in gene editing and gene regulation in different international projects. Since joining QIAGEN in 2019, Dr Martorana has been involved in developing CRISPR- and functional gene regulation-related products.
Daniel Heinz Löfgren, Market Development Manager
Daniel Heinz Löfgren is the Market Development Manager for digital PCR (dPCR) and PCR at QIAGEN for EMEA. He has over 10 years of experience in the field of PCR, qPCR and dPCR, with assay design, different workflows and applications. In addition, he has worked for 5 years as a Field Applications Specialist for droplet digital PCR and qPCR. Furthermore, he has experience working as a Genomics Applications Specialist supporting QIAGEN’s NGS workflows.
Pharma / Biopharma