The importance of rRNA removal in RNA-seq optimization
QIAseq FastSelect: Increase your unique reads, discover more biology
Did you know that a critical step for RNA-seq optimization is efficient removal of rRNA? The amount of rRNA removed is directly proportional to the number of unique gene reads obtained from your library. Efficient, near complete rRNA removal prior to sample cDNA synthesis ensures you aren't making unwanted ribosomal cDNA. Introduce our game-changing QIAseq FastSelect technology into your workflow after performing your RNA-seq library preparation protocol and experience highly efficient rRNA removal in as little as 14 minutes. In fact, samples treated with QIAseq FastSelect don't require pre-sequencing PCR amplification – simply use your library as is, save even more time and obtain a higher number of unique gene reads. FastSelect your sample before using your library preparation kit and discover more biology.
QIAseq FastSelect and SARS-CoV-2
Unravel host-microbiome interactions to advance coronavirus metatranscriptomics research
Working with mixed human and microbiome samples from nasopharyngeal, lung and other tissues for coronavirus metatranscriptomics studies? To maximize unique mRNA/gene expression reads, use QIAseq FastSelect –rRNA HMR Kits and QIAseq FastSelect –5S/16S/23S Kits separately or in combination for fast, effective and targeted removal of both host and bacterial rRNA – while still retaining viral RNAs.
Recent SARS-CoV-2 publications using QIAseq FastSelect:
Top 5 reasons why QIAseq FastSelect should be part of your RNA-seq workflow
- It’s super fast (rRNA removal in 14 min or <1 hour, depending on organism)
- It removes >95% rRNA/globin mRNA
- It improves RNA-seq sensitivity
- It enables deeper gene expression insights
- It can be used with stranded RNA-seq library kits from multiple vendors