Penta·His Antibody, BSA-free
For sensitive detection of His-tagged proteins
Monoclonal mouse Anti·His Antibodies are used to detect recombinant proteins carrying His tags. Each of the three variants detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins.
Many different proteins have been detected, via their His tags, using the QIAexpress Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of QIAexpress Anti·His Antibodies)
For other experiments with Anti·His Antibodies see figures Identification of positive expression clones by colony blotting and His-tagged thioredoxin detected by Penta·His Antibodies in yeast cells
All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody. See figure Immunohistochemistry with Penta·His Antibodies.
QIAexpress Detection Systems allow efficient detection of His-tagged proteins and are ideal for many applications, including:
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