What transfection reagents are compatible with the Cignal Reporter Assays?
Any transfection reagent that yields 25-30% transfection efficiency in the cell line of interest is suitable for use with DNA-based Cignal Reporter Assays.
Cignal Reporter Assays provide a rapid, sensitive, and quantitative assessment of signal transduction pathway activation by measuring the activities of downstream transcription factors. When conducting experiments using Cignal Reporter Assays, proper controls are a key element of the experimental design. They permit accurate interpretation of reporter assay results and provide assurance of the specificity of the observed response.