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MagAttract Viral RNA Kit

For hands-free isolation of RNA from respiratory-swab samples using automated processing and liquid handling systems

Features

  • Automated high-throughput isolation of viral RNA

  • Up to 960 respiratory human swab samples in less than one day

  • Optimized for use with the Thermo Scientific KingFisher Flex platform

Products

The MagAttract Viral RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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MagAttract Viral RNA Kit (960)

Cat. No. / ID: 955538

Automated virus preparation on a third-party platform (KingFisher Flex). Proteinase K and buffers for offboard lysis, MagAttract Suspension G beads and buffers for onboard bind, wash and elute steps.
€2,421.00
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The MagAttract Viral RNA Kit (960) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The MagAttract Viral RNA Kit combines the speed and efficiency of silica-based nucleic acid purification with the convenient handling of magnetic particles. A well-established technology is used for viral RNA preparation in a 96-well format. The purification procedure is designed to ensure reproducible handling and comprises four steps: lyse, bind, wash and elute.

Principle

The MagAttract Viral RNA Kit provides fully automated purification of viral RNA from respiratory swab samples. MagAttract technology combines the speed and efficiency of silica-based RNA purification with the convenient handling of magnetic particles. Samples are processed in the KingFisher Flex Workdeck. No phenol–chloroform or manual column extraction is required.

Procedure

In the automated workflow, viral RNA binds specifically to the magnetic beads while contaminants such as divalent cations and proteins, are completely removed in three efficient wash steps. High-quality RNA is eluted in a special RNase-free buffer, ready for direct use or safe storage. The purified RNA is free of proteins, nucleases, and other contaminants or inhibitors and can be used immediately in demanding downstream applications, including PCR, qPCR, dPCR and next-generation sequencing. Protocols are available for use on the Thermo Scientific KingFisher Flex. This kit can also be used with many other automated handling systems.

Carrier RNA

Carrier RNA serves two purposes: First, it enhances binding of viral nucleic acids to the magnetic beads, especially if there are very few target molecules in the sample. Secondly, the addition of large amounts of carrier RNA reduces the chance of viral RNA degradation in the rare event that RNase molecules escape denaturation. Not adding carrier RNA to Buffer ACL may lead to reduced viral RNA recovery.

Applications

The high-quality RNA obtained using the MagAttract Viral RNA Kit and the Thermo Scientific KingFisher Flex platform is suited for use in many downstream applications, such as:

  • Viral epidemiology and genotyping
  • Infectious disease research

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time PCR, digital PCR, genotyping, sequencing
Sample amount300 µl
For automated processingKingFisher Flex Workdeck
Elution volume80 µl
Main sample typeRespiratory swabs
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral RNA
ProcessingAutomated
Format96 well
TechnologyMagnetic particles

Resources

Kit Handbooks (0)

FAQ

Where can I download the protocol for the KingFisher Flex?

You will find a link to the protocol under the Resources tab.

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Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?

Please contact QIAGEN Technical Services.

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Who can help in case of issues with the third party instrument?

Please contact the respective QIAGEN Technical Services.

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Is the virus inactivated during the procedure?

This cannot be proven with the methods we have at our disposal.

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I would like to use the kit on another third party instrument. Who should I contact?

Please contact QIAGEN Technical Services.

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Can I use sample volumes other than 300 µl?

Using the same protocol, 200, 300, and 400 µl can be prepared, with the expected loss or gain in sensitivity.

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Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?

Not yet, but these might be added here in the future. Please contact QIAGEN Technical Services.

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