High-quality DNA isolation from various microbial cultures using automation
The MagAttract Microbial DNA Kit is designed for isolating DNA from up to 1.8 ml of culture with automated, hands-free systems. It allows for the identification of DNA from a range of microorganisms found in food and other samples. The MagAttract Microbial DNA Kit includes Inhibitor Removal Technology to remove compounds that inhibit downstream processes, including polysaccharides and lipids, leaving high-quality DNA for qPCR, Sanger sequencing and next-generation sequencing applications. Bacteria and fungi can be identified from meats, dairy products, fruits, vegetables and chocolate.
The MagAttract Microbial DNA Kit was created with automated processing and liquid handling systems in mind. QIAGEN offers protocols for the Thermo Scientific KingFisher and Eppendorf epMotion platforms, but other systems, such as those offered by Tecan, Hamilton and Beckman are also available.
For 1000 x 20 μl reactions: 12 x 0.85 ml HotStarTaq Plus Master Mix (contains 1000 units of HotStarTaq Plus DNA Polymerase, PCR Buffer with 3 mM MgCl2, and 400 μM of each dNTP), 4 x 0.55 ml CoralLoad Concentrate, 8 x 1.9 ml RNase-Free Water