STAR Q Punch AS and STAR Q Swab AS Instruments - QIAgility: Reproducible automated pipetting for your capillary electrophoresis plate setup

STAR Q AS Instruments Brochure 12/2016 11 QIAgility: Reproducible automated pipetting for your capillary electrophoresis plate setup Figure 13. The QIAgility Instrument. Figure 14. Precision and reproducibility across four QIAgility Instruments. A reaction mix was prepared manually from 5 parts Master Mix and 3 parts water. An experiment was defined in the QIAgility Setup Manager using the Rotor-Gene® SYBR® Green Kit with a total reaction volume of 25 µl, resulting in a transfer of 20 µl of ready-made reaction mix and 5 µl sample input volume. The transfers were performed into weighted 200 µl single tubes (n = 36 per instrument). The tubes were weighted again after the transfer and the weight difference taken to calculate the transferred volume. For each instrument, the pipetting precision was determined as coefficient of variation (% CV). The pipetting precision was approx. 3 % CV for all instruments tested. Figure 15. Pipetting precision. The Artel® MVS® system was employed to determine the pipetting precision of the QIAgility Instrument operated by the QIAgility Setup Manager software. Three runs including 12 data points per volume were performed on three QIAgility Instruments. The QIAgility transferred Artel solutions (selected according to the Artel MVS user manual) into the Artel buffer. For each run and volume (n = 12) the coefficient of variation was calculated to determine the pipetting precision of the QIAgility. The figure shows the mean value of the % CV values. Error bars indicate 1 x standard deviation. Pipetting variation for the 1 µl sample was around 0.07 µl. 1 2 3 4 Instrument 0 Volume transferred, CV (%) 5 4 3 1 2 10.0 7.5 1 2 5 50 100 200 20 10 Volume transferred (µl) 0.0 Measured volume, CV (%) 2.5 5.0 The QIAgility is a compact benchtop instrument that enables rapid, high-precision pipetting, making it a highly suitable automation solution to prepare your sample plates for capillary electrophoresis following PCR. 1234 12550100200

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