See what's really in your sample

See what’s really in your sample 08/2016 7 choice Overcome the limitations of conventional methods Spectral content profiling discriminates between compo- nents in complex samples. A: A pure sample of calf thymus DNA shows no significant contamination, as evidenced by the simple spectrum. B: A sample of DNA spiked with RNA was precisely analyzed and quantified. Both samples were measured on the QIAxpert DNA QIAamp application. DNA is indicated by the blue absorbance line while RNA and all detected impurities are depicted by the orange line. A gray line typically appears as a result of the sample background spectrum. Due to low sample background in this case, the gray line is not visible. A yellow line typically depicts the residual spectrum that cannot be attributed to reference profiles used in the algorithm. In this case, the yellow line is flat because no residual components could be detected. The sum of nucleic acid content, impurity and residual spectrum is represented by the black line.Read the full Application Note at www.qiagen.com/QIAxpert-QC. With classic UV/Vis measurement, the concentration calculation relies only on the absorbance at 260 nm, but both DNA and RNA in the sample can contribute to this result. In reality, the method actually measures all nucleic acids in the sample and can result in overestimation of the RNA or DNA concentration. Using a fluorescent dye-based method can help quantify only the molecule of interest, but such a method is unable to provide information regarding contaminants and other nucleic acids present. QIAxpert spectral profiling combines the advantages of both methods. The smart analysis algorithm allows easy differentiation between DNA, RNA, and other contaminants within one, dye-free measurement run. By reporting total nucleic acid and molecule of interest concentration separately and by detection and subtraction of contaminants, QIAxpert gives true insight into your sample composition. *consult webpage for full list of compatible kits And there is even more: • Measure protein via absorbance or colorimetric assay • Monitor bacterial growth • Display results on your smart device via QR code • Calibrate by yourself • Many report and export formats available ‘Universal Protocols’ Non-QIAGEN kits Dedicated for QIAGEN purification chemistry* DNA • A260 dsDNA • A260 ssDNA • Spectral profiling DNA - Spectral profiling • QIAamp • QIAsymphony • QIAquick RNA • A260 RNA • Spectral profiling RNA - Spectral profiling • PAXgene • RNeasy 6.01 5 4 3 2 1 0 230 250 275 300 325 350 375 400 425 450 Wavelength (nm) Absorbance (10mm) -0.601 4 3 2 1 0 230 250 275 300 325 350 375 400 425 450 Wavelength (nm) Absorbance (10mm) -0.54 5.4 Wide range of pre-installed methods See what’s really in your sample 08/20167 230250275300325350375400425450 230250275300325350375400425450

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