See what's really in your sample - Applications and more

6 See what’s really in your sample 08/2016 Know why QIAxpert is the instrument of A smarter solution with proprietary sample assessment features and benefits QIAxpert® – a powerful system for nucleic acid quality control Sascha Fischer1 , Per Hoffmann1 , Stefan Herms1 , Katharina Pfeifer-Sancar2 , Daniel Lehmann2 1 University Hospital Basel, Department of Biomedicine, Human Genomics, Hebelstrasse 20, CH-4031 Basel, Switzerland 2 QIAGEN GmbH, QIAGEN Str. 1, 40724 Hilden, Germany Superior linearity of concentration measurements for commercial DNA and RNA samples using QIAxpert. Top row: A dilution series of calf thymus DNA measured with the QIAxpert, Nanodrop 8000 and Qubit systems. Data shown for QIAxpert reflect total nucleic acid concentrations measured with the DNA QIAamp app. Bottom row: A dilution series of human reference RNA measured with the QIAxpert, Nanodrop 8000 and Qubit systems. Data shown for QIAxpert reflect total nucleic acid concentrations measured with the RNA RNeasy® app. Target DNA concentration (ng/µl) QIAxpert Nanodrop™ Qubit™ 0 20 40 60 80 100 120 140 Observed regression line: y = 1.0117x – 0.1316 R2 = 0.9994 Target line 140 100 120 0 80 60 40 20 Measured concentration (ng/µl) Target DNA concentration (ng/µl) 0 20 40 60 80 100 120 140 Observed regression line: y = 1.039x + 0.7469 R2 = 0.9983 Target line 140 Observed regression line: y = 0.9054x – 1.1049 R2 = 0.9912 Target line 140 100 120 0 80 60 40 20 Target RNA concentration (ng/µl) 0 100 200 300 400 500 600 Observed regression line: y = 1.0708x – 0.3635 R2 = 0.9998 Target line 600 400 500 0 300 200 100 Measured concentration (ng/µl) Target RNA concentration (ng/µl) 0 100 200 300 400 500 600 Observed regression line: y = 1.1125x + 0.7356 R2 = 0.9998 Target line 600 400 500 0 300 200 100 Measured concentration (ng/µl) Target RNA concentration (ng/µl) 0 100 200 300 400 500 600 Observed regression line: y = 1.1054x + 1.1337 R2 = 0.9988 Target line 600 400 500 0 300 200 100 Measured concentration (ng/µl) Measured concentration (ng/µl) Target DNA concentration (ng/µl) 0 20 40 60 80 100 120 140 100 120 0 80 60 40 20 Measured concentration (ng/µl) 020406080100120140 020406080100120140 0100200300400500600 0100200300400500600 0100200300400500600 020406080100120140

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