Application Guide - QIAxcel Advanced

32 QIAxcel Advanced Application Guide 10/2016 Conclusions Although many DNA markers are recorded in the Arabidopsis thaliana database, markers with differences of only several bases pairs may be difficult to analyze. We anticipate that the use of the QIAxcel system, which demonstrates superior separation capability and simplicity, will enable the effective use of these markers and further increase the speed of mapping and genotyping. References 1. Bell, C.J., Ecker, J.R. (1994) Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics, 1, 137. 2. Konieczny, A., Ausubel, F.M. (1993) A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. 4, 403. 3. Neff, M.M., Turk, E., Kalishman, M. (2002) Web-based primer design for single nucleotide polymorphism analysis. Trends Genet, 18, 6135. Figure 2. Assessment of ecotypes based on the NGA707 marker. Col H Ler 5386 – 587 – 498 – 298 – 257 – 174 – 102 – 15 – – 434 – 267 – 80 bp Figure 4. Examination of mutations based on dCAPS. +/+ +/– –/– 5386 – 1078 – 603 – 234 – 118 – 15 – GUN5: bp Figure 3. Predicted sizes of restriction digested wild-type and mutant fragments. WT gun5–1 54 54 146 178 32 Primer 1 Primer 2

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