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Application Guide - QIAxcel Advanced

QIAxcel Advanced Application Guide 10/2016 125 For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor. Discover the benefits of automated gel electrophoresis at www.qiagen.com/QIAxcel. Trademarks: QIAGEN® , QIAsymphony® , QIAxcel® , Sample to Insight® , ScreenGel® (QIAGEN Group). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. Ordering www.qiagen.com/shop Technical Support support.qiagen.com Website www.qiagen.com References 1. Council Regulation (EC) No 510/2006 of March 20, 2006 2. Colombo, F. et al. (2002) Identification of the goose species (Anser anser) in Italian “Mortara” salami by DNA sequencing and a polymerase chain reaction with an original primer pair. Meat Sci. 61(3), 291. 3. Matsanuga, T. et al. (1999) A quick and simple method for the identification of meat species and meat products by PCR assay. Meat science 51, 143. 4. Schwägele, F. et al. (2005) Traceability from a European perspective. Meat Sci. 71(1), 164 5. Wolf, C. et al. (1999) PCR-RFLP analysis of mitochondrial DNA: a reliable method for species identification. J. Agric. Food Chem. 47, 1350. 6. Meyer, R. et al. (1995) Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis: A simple method for species identification in food. J. of AOAC Int. 78(6), 1542. 7. Pascoal, A. et al. (2004) Survey of authenticity of meat species in food products subjected to different technological processes, by means of PCR-RFLP analysis. Eur. Food Res. Technol. 218, 306. 8. Maede, D. (2006) A strategy for molecular species detection in meat and meat products by PCR-RFLP and DNA sequencing using mitochondrial and chromosomal genetic sequences. Eur. Food Res. Technol. 224, 209. Ordering Information Product Contents Cat. no. QIAxcel Advanced System Capillary electrophoresis device: includes computer, QIAxcel ScreenGel software, and 1-year warranty on parts and labor 9001941 QIAxcel DNA High Resolution Kit (1200) QIAxcel DNA High Resolution Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, 12-Tube Strips 929002 Buffer ATL (4 x 50 ml) 4 x 50 ml lysis buffer for use in purification of nucleic acids using the QIAsymphony DSP DNA Mini Kit 939016 QIAsymphony SP QIAsymphony sample prep module 9001297 QIAsymphony DSP DNA Mini Kit (192) For 192 preps of 200 ul each: Includes 2 reagent cartridges and enzyme racks and accessories. 937236 TopTaq Master Mix Kit For 200 x 50 ul reactions: 2x TopTaq Master Mix containing 250 units of TopTaq Polymerase in total, 10x CoralLoad Concentrate, and RNase-Free Water 200403 QX Alignment Marker 15 bp/600 bp (1.5 ml) Alignment marker with 15 bp and 600 bp fragments 929530 QX DNA Size Marker 25–500 bp (50 ul) v2.0 DNA size marker with fragments of 25, 50, 75, 100, 150, 200, 250, 300, 400, and 500 bp; concentration 100 ng/ul 929560

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