Helping you unveil adaptive immune responses with an all-in-one solution
Next-generation sequencing (NGS) can provide an unprecedented, high-resolution picture of the T-cell receptor (TCR) repertoire. However, automating NGS workflows for this purpose can be challenging, as the multiplex PCR required for targeting different V or J regions can introduce substantial amplification bias.
Our convenient Immune Repertoire Profiling Services use a highly efficient, TCR-specific cDNA synthesis reaction to enrich the complete TCR variable region with gene-specific primers targeting TCR-constant regions and a universal primer complementary to the ligated adapter. Amplification uniformity is superior to amplification with multiplexed V and J primer pools. Amplification bias is further reduced using unique molecular indices (UMIs). The result of these combined technologies is accurate and sensitive TCR clonotype and repertoire diversity assessment.
Our Immune Repertoire Profiling Services offer:
- End-to-end service: We take care of every step, from sample preparation to data analysis
- Guidance and flexibility: We aid in designing your project and help you make the right decisions
- Optimized TCR variable region enrichment: We use unique molecular indices to reduce amplification bias, providing accurate and sensitive clonotype and repertoire diversity assessment
- Ready-to-publish data: We deliver comprehensive reports and data packages including frequency and identity for each unique clonotype, V and J segment usage, CDR3 length distribution and diversity metrics
Our comprehensive Immune Repertoire Profiling workflow