QIAfilter Plasmid Giga Kit
Transfection grade의 plasmid 혹은 cosmid DNA를 10 mg 까지 빠르게 분리할 수 있습니다
QIAfilter Plasmid Kits provide anion-exchange-based plasmid DNA purification with bacterial lysate clearing by filtration. The kits deliver DNA yields of up to 10 mg. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.
QIAfilter Plasmid Giga Kit are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
The QIAfilter Plasmid Giga Kit combines QIAfilter Mega-Giga Cartridges for rapid clearing of bacterial lysates by filtration instead of centrifugation, with proven QIAGEN-tip 10000 anion-exchange tips for efficient plasmid purification. Using the vacuum-operated QIAfilter Mega-Giga Cartridge and the QIAGEN-tip 10000, up to 10 mg transfection-grade, high-copy plasmid DNA are purified from 2.5 liters culture. (Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.)
For purification of low-copy plasmids and cosmids, QIAfilter Plasmid Mega Kits are a better choice than QIAfilter Plasmid Giga Kits, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge.
QIAfilter Cartridges, provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour.
QIAfilter Mega-Giga Cartridges operate with house vacuum to efficiently clear even large volumes of bacterial lysate with minimal effort.
The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.
Neutralized bacterial lysates are incubated directly in the QIAfilter Cartridge and cleared rapidly by filtration. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer (see flowchart "QIAGEN Plasmid Kit procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.
DNA obtained with QIAfilter Plasmid Kits provides excellent results in all applications, from cloning to transfection.
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