artus HAV TM RT-PCR Kit v2.0 RUO

Real-time PCR을 이용하여 hepatitis A virus RNA를 정량 검사할 수 있습니다

S_1084_5_GEN_V2

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artus HAV TM RT-PCR Kit v2.0 (24) RUO

Cat. No. / ID:  4516103

For 24 reactions: 2 Masters, 4 Quantitation Standards, Internal Control, Water (PCR grade)
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연구 전용. 진단 절차에는 사용할 수 없습니다. 모든 주장이나 진술은 질병의 진단, 예방 또는 치료를 위한 정보를 제공하기 위한 것이 아닙니다.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 0.1 IU/ul만큼 적은 샘플의 고감도 검출
  • Internal control을 이용하여 신뢰할 수 있는 결과 얻어냄
  • 제공되는 4개의 standard를 이용하여 viral load를 정확하게 정량 할 수 있음

Product Details

The artus HAV TM RT-PCR Kit v2.0 is a ready-to-use molecular detection kit for real-time PCR on ABI PRISM 7000, 7700, and 7900HT Sequence Detection Systems. The kit provides all necessary reagents optimized for rapid and sensitive detection of HAV RNA from human serum and plasma samples with highly accurate quantification.

Performance

To ensure sensitivity, the artus HAV TM RT-PCR Kit v2.0 has been optimized to detect low numbers of HAV RNA. The analytical sensitivity of the kit is 0.2 IU/μl in the PCR on the ABI PRISM 7000 SDS and 0.1 IU/μl in the PCR on the ABI PRISM 7700 SDS and the ABI PRISM 7900HT SDS.

For reliable specificity, testing of the artus HAV TM RT-PCR Kit v2.0 was carried out using various HAV strains, including all relevant genotypes (IA, IB, II, IIIA, VII), and related pathogens.

Performance of artus HAV TM RT-PCR Kit v2.0
Kitartus HAV TM RT-PCR Kit v2.0
Sample types Serum or plasma
Analytical sensitivity 0.2 IU/µl (ABI PRISM 7000 SDS); 0.1 IU/µl (ABI PRISM 7700 SDS and 7900HT SDS)
 Specificity  HAV, including genotypes IA, IB, II, IIIA, and VII

Principle

The artus HAV TM RT-PCR Kit v2.0 is based on the amplification and simultaneous detection of a highly conserved region within the HAV genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

The artus HAV TM RT-PCR Kit v2.0 provides 4 HAV quantitation standards (see figure " Reliable quantitation of HAV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical HAV RT-PCR is not reduced.

Specifications of artus HAV TM RT-PCR Kit v2.0
Kitartus HAV TM RT-PCR Kit v2.0
Sample types Serum or plasma
Amplicon 90 bp region of the HAV genome
See figures

Procedure

The artus HAV TM RT-PCR Kit v2.0 provides all necessary reagents optimized for reliable HAV RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, we recommend using the QIAamp UltraSens Virus Kit, QIAamp Viral RNA Mini Kit, QIAamp DSP Virus Kit, or QIAamp DNA Stool Mini Kit.

Applications

The artus HAV TM RT-PCR Kit v2.0 allows the rapid and sensitive detection of HAV RNA from serum and plasma. The kit is available for use on ABI PRISM 7000, 7700, and 7900HT Sequence Detection Systems.

Supporting data and figures

Specifications

FeaturesSpecifications
Quantitative/qualitativeQuantitative
Recommended sample prepDepending on the starting material and sensitivity needs: QIAamp Ultrasens Virus Kit, QIAamp Viral RNA Mini Kit, QIAamp DSP Virus Kit, QIAamp DNA Stool Mini Kit
RUO/CE/ASRRUO
Thermal cyclerABI PRISM 7000, 7700, 7900HT SDS
Sample typePlasma, serum, fecal suspensions
What detectedHepatitis A Virus RNA

Resources

Kit Handbooks (1)
For use with the ABI PRISM 7000, 7700 and 7900HT Sequence Detection Systems
Certificates of Analysis (1)