EZ1&2 DNA Kits

EZ1&2 DNA Kits contain all required reagents and labware for rapid, automated purification of DNA from up to 200 μl blood samples, up to 350 μl blood or buffy coat samples or up to 40 mg tissue samples using magnetic-particle technology. Reagents are supplied in prefilled and sealed reagent cartridges, which ensures speed and convenience in loading the instrument.

EZ1&2 DNA Kits are automated with the EZ2 Connect. These kits can also be used with the EZ1 Advanced XL (1–14 samples per run).

The automated procedure provides high repeatability (see figures  "High-quality DNA from whole blood" and  "Reproducible yields of high-quality DNA from whole blood") and linear yields over a range of starting amounts. DNA purified using EZ1&2 DNA Kits gives high-performance results in downstream applications, such as PCR (see figures  "High-quality DNA for sensitive and specific analyses" and  “Consistent PCR results”).

Automated purification with EZ1&2 DNA Kits requires use of either EZ2 Connect instruments or EZ1 instruments and EZ1 DNA Cards (see table “EZ1&2 DNA Kits and EZ1 DNA Cards”).

EZ1 Cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination.

EZ1&2 DNA Kits and EZ1 DNA Cards

Tissues are first incubated in Buffer G2 containing proteinase K until lysed and then homogenized. Whole blood samples (200 µl or 350 µl) and pretreated tissues are loaded onto the instrument. Genomic DNA is then purified in one step following the automated EZ1&2 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl or 50 µl volumes, ready to use in all downstream applications, such as PCR.

The high-quality DNA obtained using EZ1&2 DNA Kits is suited for use in many applications, such as:

• Population genetics and pharmacogenomic research
• PCR and real-time PCR
• Sequencing
• Oncology research
• Genetic identity testing and forensics
• Genotyping analysis, including STR, VNTR, RAPD, NASBA, SNP and AFLP technologies