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Next-generation sequencing

Unravel the complexity of the genome with DNA-seq solutions

Overview

Whether you’re interested in detecting DNA variants or identifying known and novel RNA fusions, complex workflows, long turnaround times and limited samples can impede progress, increase costs and delay actionable data. Our high-performance DNA sequencing (DNA-seq) technologies are optimized to overcome such challenges so you can sequence GC-rich regions with confidence, identify low-frequency variants with precision and get the most out of low-quality or limited-input samples. If you need custom panels, our experts are on hand to help with intelligent panel design.

Genomic insights powered by QIAseq

QIAseq solutions are based on the following innovations to minimize bias and maximize genome coverage, uniformity and precision.

Amplicon sequencing

Redefine amplicon sequencing: Find the variant that matters with targeted DNA-seq

PCR duplicates, false positives and library bias can undermine the quality of your targeted sequencing data. Introduce precision and confidence into your workflow by adopting the digital DNA sequencing approach.

Our platform-agnostic, expertly curated, targeted DNA sequencing panels utilize unique molecular indices to ensure superior coverage of:

GC-rich genomic regions
Exonic regions of genes
Hotspots
SNPs
Intronic and promoter regions

FIND THE PANEL YOU NEED

Error or low-frequency mutation?

Targeted DNA sequencing with panels is a powerful approach to detect low-frequency variants. However, a major drawback is that all DNA fragments look exactly the same, making it almost impossible to distinguish sequencing errors and unique DNA molecules. This limits your ability to detect low-frequency DNA variants confidently. Not with QIAseq – all catalog and custom panels contain unique molecular indices, which tag the original DNA molecules and eliminate false reads due to PCR, polymerase and machine-read errors.

Access the most difficult regions of the genome

QIAseq products include optimized amplicon sequencing buffers and enzymology, allowing you to access the most difficult regions of the genome. The chemistry used in the QIAseq Targeted DNA panels and workflow is compatible with both regular and GC-rich genomic regions. Achieve 100% coverage for genes rich in GC content such as CEBPA and CCND1 using tiling methods.

Outstanding sequencing metrics

Uniformity: Standard panels typically achieve uniformity of 99.5% at 0.2x of mean coverage and 96% at 0.5x of mean coverage.

Sensitivity: Gain higher confidence in calling low-frequency DNA variants. Standard panels can obtain over 90% sensitivity for 1% NA12878 SNP and indel on A typical coding region with false positives of less than 15 per megabase region.

More multiplexing: Products from other suppliers offer 96 sample multiplexing. QIAseq does not stop there: 384 samples can be multiplexed using indices specific to QIAseq.

Featured success story

"QIAseq panels are able to get into difficult regions of the genome because they utilize a single primer extension design strategy. Up to 1500 samples can be sequenced simultaneously."

Dr. Fergus Couch, Mayo Clinic

QIAseq custom panels drive new insights into pancreatic cancer

Performing NGS in oncology research and looking to maximize coverage and access the most difficult regions of the genome? Then our QIAseq NGS panels may be just the tool you need to achieve impactful insights! A recent longitudinal case-control study on the risk of pancreatic cancer – led by Dr. Fergus Couch from the Mayo Clinic – illustrated the efficacy of our QIAseq Targeted DNA Panels in ensuring 99.7% coverage of target regions. Interested in applying the unique benefits of our QIAseq Targeted DNA Custom Panels your own research? Have a look at the details of the Mayo Clinic study for inspiration!

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Single primer extension vs. the two-primer amplicon approach

A head-to-head comparison of targeted sequencing methods reveals why the single primer extension (SPE) method comes out on top.

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Resolve your panel design dilemmas

Frustrated by difficult content choices for NGS panel design? Worried about high GC content? When it comes to panel design, we can ensure your custom panels meet the following criteria to best target the region of interest:

Maximum coverage
Minimum bias
Uniformity

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A single unified solution for NGS and data analysis

Combine the precision of our QIAseq DNA Panels with intuitive bioinformatics tools. QIAseq DNA panels now include subscription licenses for CLC Genomics Workbench and QIAGEN Clinical Insight (QCI)-Interpret for QIAseq software, so you can make the leap from generating data to accumulating insights with greater ease and speed.

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Reduce sample mix-up and maximize sequencing throughput

Unique Dual Indices (UDI) provide the highest level of process safety and ensure confidence in sequencing data by mitigating “index hopping” and misassignment of reads. UDIs allow you to maximize sequencing throughput for lower per-sample sequencing costs. The combination of both UMIs and UDIs means that QIAseq Targeted Panels provide an unprecedented level of accuracy.

Whole exome sequencing

Sample to disease insights in record time

One optimized exome sequencing pipeline with reduced turnaround times to get you from sample to standardized variant classification and interpretation.

Automatable and scalable exome sequencing with a single-day workflow

Examining the exome to identify disease-causing variants but worried about coverage uniformity due to GC composition?

Detect pathogenic variants with confidence, ease and efficiency with our scalable single-day, automation-compatible workflow. Based on hybrid capture technology, QIAseq Human Exome Kits ensure:

Highly sensitive variant calling of targets of >500 genes
Reduced sequencing costs per sample by 50%
A 40% decrease in overall turnaround time from sample to data

Seamless integration with QIAGEN CLC Genomics Workbench enables rapid variant calling and QCI Translational ensures accurate variant interpretation and disease-specific insights – without the burden of manual curation.

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Whole genome sequencing

Capture a comprehensive view of the genome

Whole genome and hybrid capture sequencing provide a holistic view of the entire genome and unparalleled insights, but they can be time-and cost-intensive approaches. QIAseq solutions make these methodologies more accessible by maximizing per-sample data quality.

Benefit from:

Advanced chemistries for picogram sample input and fast, automatable protocols
Enzymatic DNA fragmentation – no need for expensive mechanical shearing equipment
Increased ligation efficiencies for high conversion rates and yields – no adapter-dimers
Uniform G/C representation from any organism
Convenient kit formats with included dual-barcoded adapters

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Featured success story

Dr. Egbert Schulze from the Endocrinology and Nuclear Medicine Community Practice, Heidelberg, discusses how the all-enzymatic QIAseq FX workflow enabled uniform coverage and helped him achieve reliable sequencing results in his research.

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Automate and accelerate your library prep

How can automating your NGS library prep benefit your lab?

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Single-cell DNA-seq

PCR-free, single-cell whole genome library prep with comprehensive coverage and high sequence fidelity

Are single cells or limited genomic DNA amounts limiting your ability to gain a deeper view of the genome? Explore how you can prepare whole genome libraries from single cells in <4 h using our single-cell DNA library prep kit. The PCR-free protocol eliminates bias, so you can confidently analyze aneuploidy, copy number and sequence variation in single cells from rare samples.

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Metagenomics

Deciphering microbial diversity

Metagenomic data is used to examine the species present in natural or healthy microbiomes, quantify the abundance of virulent or antibiotic resistance genes, or shed light on the causes, effects and future therapies for a variety of diseases. From PCR amplicon sequencing to whole metagenomic analysis of a DNA sample, QIAseq NGS solutions are designed to unravel the genomic complexity of your sample.

FIND THE RIGHT TOOLS

Check out the Workflow Configurator

This smart online tool can help you find the right products for every step of your workflow.

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Webinar highlights

Enterprise Genomics Solutions

Combine unparalleled expertise and support with our high-performance QIAseq technologies

Be part of the genomic revolution! Partner with QIAGEN to benefit from >20 years of experience in Sample to Insight solutions. From panel design to analysis and beyond – our Enterprise Genomic Solutions simplify biomarker discovery to propel your research forward.

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All photos taken prior to COVID-19