Liquid biopsy workflow | QIAGEN

Like you, we believe liquid biopsy has huge potential. However, getting meaningful insights from analytes in biofluids can be difficult.
To help you navigate your way from sample collection to data interpretation, we have designed a step-by-step guide for your entire workflow.

Paving the way for liquid biopsy

1. Collection & stabilization

How you collect and preserve your biofluid samples will impact the final result.
Avoid variation in quality and integrity using our robust PAXgene Blood ccfDNA Tubes - designed to reliably stabilize both analyte and cell integrity without negatively impacting your sensitive downstream assays.

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2. Sample preparation

Extracting analytes from liquid biopsy sample can be challenging due to low analyte abundance, a high level of background and risk of degradation.
Using over 30 years’ experience in sample preparation, we have created a wide selection of specialized automated and manual tools to enrich and extract circulating cell-free DNA (ccfDNA), circulating tumor cells (CTCs), exosomes, cell-free miRNA or multianalyte from the most important biofluids.
Sample preparation
  • Purify ccfDNA
    Explore our ccfDNA portfolio to get the highest yields and purity of ccfDNA from a range of starting materials. With scalable input volumes and standardized automated workflows, you also get unmatched flexibility and increased productivity.
  • Characterize CTC​
    Efficiently enrich and characterize CTCs from blood using our two-step AdnaTest system. The first step uses an optimized antibody mixture for highly specific immunomagnetic selection. The second step allows sensitive gene expression analysis.
  • Extract exosomes​
    Enjoy spin column simplicity with ultracentrifugation quality with our exoRNeasy and exoEasy Kits. Specifically developed for fast and convenient extraction of exosomal RNAs and intact exosomes from large volumes of biofluids.
  • Purify cell-free miRNA
    Get safe, convenient purification of total RNA from serum and plasma using our miRNeasy Serum/Plasma Advanced Kit. By combining easy-to-automate MinElute spin-column technology with a phenol-free protocol, you get consistent, hassle-free high-quality yields.
  • Purify ccfDNA/ccfRNA
    Uncover a comprehensive picture of the transcriptome and genome by simultaneously isolating excellent yields and quality of cell-free DNA and RNA from the same sample using our QIAamp ccfDNA/RNA Kit.

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3. Detection

Choosing the best platform to analyze your samples requires careful consideration.
If you are interested in capturing all sequences and finding new targets, we have a broad selection of NGS solutions for you to explore.
For interrogating known targets and confirming NGS results, we provide highly sensitive and specific qPCR technologies.
  • Targeted NGS
    Detect low-frequency variants with high confidence by overcoming the issues of PCR duplicates, false positives and library bias using our QIAseq Targeted DNA panels.
  • miRNA sequencing
    Eliminate adapter dimers and unwanted RNA species for greater sequencing efficiency using the QIAseq miRNA Library Kit.
  • RNA biomarker profiling
    Obtain complete gene expression profiles by simultaneously analyzing mRNA, miRNA and lncRNA using our range of designed and custom miRCURY and RT2 panels and arrays.
  • CTC gene expression​
    Achieve the highest degree of analytical specificity and sensitivity in CTC biomarker detection and discovery using highly sensitive AdnaTest RT-PCR technology.

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4. NGS data analysis

Data analysis
Finding differentially expressed genes in a vast number of mutations can be overwhelming. Overcome this, using our CLC Genomics Workbench - a powerful solution to analyze and visualize your NGS data. By combining cutting-edge technology and accelerated algorithms, you get a comprehensive set of NGS data analysis applications.

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5. Data interpretation

Turning your data into biologically relevant insights requires advanced bioinformatics tools.
Discover a comprehensive, user-friendly platform for interpreting your ‘omics data with Ingenuity Pathway Analysis.
Liquid biopsy data interpretation

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