PyroMark Q24 and PyroMark Q24 Advanced

For reagents and support of discontinued instruments PyroMark Q24 and PyroMark Q24 Advanced.

S_2018_ADNA_PyroM001_s

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PyroMark OneStep RT-PCR Kit (50)

Cat. No. / ID:  978801

For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water
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KitAssayAccessoriesReagentSoftware
PyroMark Kits
Assays and Tests
Buffers
Q24 Reagents
Consumables
PyroMark Q24 Software
PyroMark Software
Type
OneStep RT-PCR Kit
PyroMark PCR Kit
Reactions
50
200
The PyroMark Q24 Instrument and PyroMark Q24 Software and PyroMark Q24 Advanced Instrument and PyroMark Q24 Advanced Software are intended to be used only in combination with QIAGEN kits indicated for use with the PyroMark Q24 and PyroMark Q24 Advanced for the applications described in the kit handbooks. If the PyroMark Instruments and Software are used with other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Consider upgrading to a PyroMark Q48 Autoprep System for these advantages

  • Automation of the Pyrosequencing protocol without the need for a manual process on a vacuum prep workstation
  • Up to 48 samples can be analyzed
  • Compact instrument size
  • Enabled for connectivity
  • Touch screen display that guides users through the process

Product Details

The PyroMark Q24 was discontinued in 2018. The instrument is no longer sold, but existing instruments will be supported until the end of 2025. In addition, reagents will continue to be available until the end of 2025.

To future-proof your Pyrosequencing assays, consider upgrading your work to using a PyroMark Q48 Autoprep System which is actively sold and supported by QIAGEN.

Service Plans

Pyro Q24, Full Agreement

Cat. No. / ID:  9241822

Instrument repair service for the PyroMark Q24 at regional repair center. Shipping, labor and parts included during the Full Agreement period. Loaner instrument provided within 2–3 business days. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service during the Full Agreement period.
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Pyro Q24, Preventive Subscription

Cat. No. / ID:  9243547A

One on-site Preventive Maintenance or Inspection Service visit for the PyroMark Q24, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.
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Pyro Q24, Core Agreement

Cat. No. / ID:  9245245

One-time PyroMark Q24 instrument repair performed at a regional repair center including shipping, labor, and spare parts for a period of one year. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service and a 10% discount on additional repair service during the Core Agreement period.
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Resources

Download Files (28)
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0011
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0012
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0007
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0015
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0014
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0008
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0008
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0013
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0004
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0002
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0004
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0009
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0011
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0005
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0010
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0005
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0013
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0003
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0012
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0001
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0006
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0010
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0006
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0009
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0003
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0007
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0014
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0015
Operating Software (4)
This version is compatible with Windows 7 and Windows 10 (64 bit) operating systems. This software may only be downloaded by registered users with a valid PyroMark Q24 software license and registered PyroMark Q24 instrument. If you do not have a valid software license, contact your QIAGEN sales representative.
Files for upgrading existing PyroMark Q24 instruments to firmware 1.5.6903
PyroMark Q24 Advanced Software version 3.0.1 is compatible with Windows 7 and Windows 10 (64 bit) operating systems. This software may only be downloaded by registered users with a valid PyroMark Q24 Advanced Software license and registered PyroMark Q24 Advanced instrument. If you do not have a valid software license, contact your QIAGEN sales representative.
PyroMark Assay Design Software version 2.0.2 is compatible with Windows 7 and Windows 10 (64 bit) operating systems. This software may only be downloaded by registered users with a valid PyroMark Assay Design Software license. If you do not have a valid software license, contact your QIAGEN sales representative.
Kit Handbooks (8)
For performing Pyrosequencing reactions on the PyroMark Q24
For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems
October 2009
Software User Guides (1)

FAQ

How do I retrieve a backup copy if the data file on the USB stick cannot be opened from PyroMark Q24 or PyroMark Q24 Advanced instruments?

Do not remove the USB stick while data are being copied. Corrupted data files are usually due to the interrupted copying process. Follow these steps to retrieve recently saved runs.

 

1. When the instrument is not processing, insert a USB stick into the USB port on the instrument.

 

2. Use the Up and Down buttons to select “Administration” in the menu and press OK.

 

3. Select “Copy Recently Saved Runs” and press OK.

 

4. Use the Up and Down buttons to select the run file(s) for retrieval and press Select.

 

5. When the instrument confirms that the run file(s) has been saved to the USB stick, press Close and remove the USB stick.

 

In addition, repeat the process with a different USB stick. If the issue persists, please contact QIAGEN Technical Service for further assistance.

FAQ ID -9053
Which operating system is compatible with PyroMark Q24 Advanced application software?

The new PyroMark Q24 Advanced Software (version 3.0.1) is compatible with Windows XP, Vista (32 and 64 bit), Windows 7 (32 and 64 bit), and Windows 10 (64 bit).

FAQ ID -3164
Can I order the nucleotides from PyroMark Gold Reagents separately?
The nucleotides can only be ordered as part of the PyroMark Gold Reagents which also contain enzyme and substrate mix.
FAQ ID -2827
Can I use the PyroMark Gold Q24 reagent on the PyroMark Q24 Advanced system or vice versa?

PyroMark Gold Q24 reagent and the PyroQ24 Advanced reagent can only be used on the intended instrument model. The chemistry in the PyroMark Q24 Advanced reagent has been improved. Using PyroMark Q24 reagent on a PyroMark Q24 Advanced instrument can lead to much lower peaks; while using PyroMark Q24 Advanced reagent on a PyroMark Q24 instrument can result in increased background and running out of enzyme and substrate prematurely.

FAQ ID -9070
How much space does the PyroMark Q24 instrument need?

PyroMark Q24 takes very little bench space, measuring only H420 x W390  x D525 mm.

 

FAQ ID -2098
Can I run multiple types of assays in one run?

Yes. The PyroMark Q24, PyroMark Q24 Advanced, and PyroMark Q96 v2.5 software provide multiple types of assay modes, which enable analyzing different types of samples at the same time.

FAQ ID -9057
How do I validate a new Pyrosequencing assay?

All new assays have to be validated by the user. Interaction between primers or loops formed on single-strangled DNA can serve as priming sites for base incorporation by DNA polymerase. The following controls should be included when an assay is analyzed for the first time:

 

 1. PCR without template DNA —shows if the primers interact to give a background signal in Pyrosequencing reactions

 

2. PCR with template DNA but with no sequencing primer — shows if the template can loop back on itself and give a background signal in Pyrosequencing reactions

 

3. Sequencing primer without any PCR product — shows if the sequencing primer can form duplexes or hairpins and give background signal in Pyrosequencing reactions

 

4. Biotinylated primer without any PCR product — shows if the biotinylated primer can form duplexes or hairpins and give background signal in Pyrosequencing reactions

 

5. Sequencing primer and biotinylated primer together without PCR product —shows if the sequencing primer and the biotinylated primer can form duplexes and give background signal in Pyrosequencing reactions

 

Programs from these controls ought not to show any significant peaks after any nucleotide addition.

FAQ ID -9066
How-can-the-histogram-help-trouble-shooting-a-Pyrosequencing-run?

The histogram is the theoretical peak pattern based on the Sequence to Analyze that you enter into the software, while the pyrogram is the experimental peak pattern that is detected by PyroMark instruments. The peak pattern in the pyrogram has to be in agreement with that in the histogram for a successful run. Overlaying the histogram on the pyrogram provides an invaluable tool for trouble-shooting Pyrosequencing runs. To do so, right click anywhere on the pyrogram and select Show Histogram on the pop-up menu.

FAQ ID -9056
Which analyses can be performed with the PyroMark Q96 ID software version 1.0?
The PyroMark Q96 ID software version 1.0 contains a SNP mode for simplex and multiplex entries (and Allele Quantification analyses can be performed for simplex entries) and an SQA mode for de novo sequencing analysis. This software version does not contain a CpG mode for methylation analysis, however the PyroMark CpG software v1.0 can be used.
FAQ ID -2845
Does the PyroMark CpG LINE assay target mouse transposons as well?

The PyroMark CpG LINE-1 assay is specific for human DNA and was not tested on mouse DNA.  Mouse LINE-1 elements differ slightly in sequence and may not be detected.

 

 

-1
What causes peaks to appear between dispensations on the pyrogram?

This can result from very high signal in a well which results in cross-talk between neighbouring wells. It could also be that the PyroMark instrument's camera is misaligned (contact: QIAGEN Technical service).

FAQ ID -9060
Which analyses can be performed with the PyroMark Q96 MD software?

The PyroMark Q96 MD software contains a SNP mode for simplex and multiplex entries (and Allele Quantification analyses can be performed for simplex entries). This software version does not contain a CpG mode for methylation analysis, however the PyroMark CpG software v1.0 can be used. SQA analysis for de novo sequencing is not possible. 

See our Product Selection Guide for additional information on CpG supplementary software.

FAQ ID -2866
What is the use of the PyroMark control oligo?

The PyroMark Control Oligo is a self-priming oligo which can be used directly in the pyrosequencing reaction without an extra sequencing primer. It was designed for installation of the different PyroMark systems and can be used for general troubleshooting.

It can be used directly for sequencing without previous PCR setup and sample preparation by the vacuum prep tool. By this test, the performance of the instrument can be checked.

In addition, it can also be processed through the vacuum sample preparation to check the performance of this procedure. The control oligo run results in a defined pyrograms with certain peak heights for the individual instruments which are described in the handbook.

The PyroMark Control Oligo can be used on all PyroMark systems including the Q24Q24 advanced, Q96 ID, and Q96 MD.

FAQ ID -9067
How can I rescue a run due to my mistake or instrument failure?

In the case of mistakes and instrument failure, the single stranded DNA can be re-used without re-run PCR. Perform the vacuum prep steps with the streptavidin beads and release them to a new Pyrosequencing plate for a fresh run.

FAQ ID -9063
What ought I do if mutation assays designed by QIAGEN fail?

Mutation assays designed  by QIAGEN detect the most common mutations. In addition, QIAGEN provides plug-ins to analyze more mutations and convenient reports for PyroMark KRAS, PyroMark EGFR, and PyroMark BRAF kits. The plug-ins can be obtained by emailing pyro.plugin@qiagen.com

 

If the mutation cannot be analyzed successfully, please send the original run files to QIAGEN Technical Service for further assistance.

FAQ ID -9065
How do I analyze unexpected mutations?

Pyrosequencing assay can detect unexpected/unknown mutations. Modify the Sequence to Analyze to analyze unexpected/unknown mutations. See How can I modify the Sequnce to Analyze post a run.

                          

If the mutation cannot be analyzed successfully, please send the original run files to QIAGEN Technical Service for further assistance.

FAQ ID -9064
What are the operating system and hardware requirements for PyroMark software?
Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?

If you need to install the PyroMark Q24 software on a new computer replacing your old one, or after you reinstall or upgrade the operating system, you can reinstall the PyroMark Q24 software without purchasing a new license.

FAQ ID - 3473

What causes low peak signals in Pyrosequencing?

Insufficient amount of PCR template, loss of streptavidin bead during vacuum prep steps, incorrect instrument method, and incorrect reagent are the common causes of low peak signals.

 

Check the PCR product with QIAxcel instrument or agarose gel electrophoresis and determine the appropriate amount of PCR product to be used. Perform vacuum prep function test. Determine the vacuum prep station functionality using the PyroMark Control Oligo with and without the vacuum prep steps. Ensure the correct instrument method is used. Refer to Managing PyroMark Instrument Methods. Ensure the correct reagent is used for the specific instrument model.

FAQ ID -9069
What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?
The new PyroMark Assay Design Software Version 2.0 contains new algorithms to facilitate CpG assay design and enables PCR and sequencing primer design for all pyroseqeuncing applications and PyroMark instruments.
FAQ ID -2849
Why must I update the instrument method when lot numbers of the cartridge/tips change?

PyroMark instruments dispense the correct volume of enzyme mix, substrate mix, and nucleotides using variable pulse times and dispensing pressures. The settings for pulse times and dispensing pressure are specific for each lot of PyroMark dispensing tips and cartridges and need to be updated in the PyroMark software being used to operate the instrument.

 

Dispensing pressures and pulse time settings must be checked and, if necessary, updated every time a cartridge from a new lot number is used. Check the details at Managing PyroMark Instrument Methods.

FAQ ID -9055
What can I expect for the reading length on various PyroMark Instruments
What causes wide peaks?

Sodium hydroxide carry-over, too much template, and incorrectly stored reagent are the most common causes of wide peaks. Ensure correct volume of denaturation buffer is used on the vacuum prep station. Use 5–20 µl of PCR product depending on instrument models. Store reagent as described in the handbook.

 

If the issue persists, please send the run files (see Instruction for run file export) to QIAGEN Technical Service for further assistance.

FAQ ID -9062
What data are required for trouble-shooting?

For PyroMark Q24, PyroMark Q24 Advanced, PyroMark Q96 ID v2.5, and PyroMark CpG software, data are file-based. The original run files can be attached to email and sent to QIAGEN Technical Service.

 

For PyroMark Q96 ID 1.0, PyroMark Q96 MD/MDA 1.0, PSQ MA, and PSQ HS/HSA software, data are stored in an Oracle database and need exporting (See Instruction for run file export). Exported data can be attached to email and sent to QIAGEN Technical Service.

 

The log files from the PyroMark Q96 ID, PyroMark Q96 MD/MDA, PSQ MA, and PSQ HS/HSA instruments provide valuable information. See Instruction for log file retrieval) for log file retrieval instruction

 

Please send the log file along with the original run file to QIAGEN Technical Service.

FAQ ID -9051
What is a single peak height? What are the optimal single peak heights on various PyroMark instruments?

A single peak height is the signal produced from a non-variable single nucleotide.  The single peak height is an important criterion for trouble-shooting.

 

The optimal single peak height is 30 RLU (minimum 20 RLU) (Relative Light Unit) for PyroMark Q24 and PyroMark Q24 Advanced, 20 RLU (minimum 10 RLU) for PyroMark Q96 ID, and 100 RLU (minimum 50 RLU) for PyroMark Q96 MD instrument, respectively.

FAQ ID -9058
Can I install the PyroMark Q96 ID 1.0, PyroMark Q96 MD/MDA 1.0, PSQ MA, and PSQ HS/HSA software on an office computer for data analysis?

The PyroMark Q96 ID 1.0, PyroMark Q96 MD/MDA 1.0, PSQ MA, and PSQ HS/HSA software requires a functional Oracle database, which cannot be installed on an office computer. You can install the PyroMark software on an office computer and access the Oracle database on the PyroMark operator’s computer via network. The operator’s computer needs to be powered on while you are accessing the Oracle database remotely. Your IT department is responsible for the network setup.

 

FAQ ID -9059
Can PyroMark Gold reagents be vortexed?
Reconstiuted enzyme and substrate of PyroMark Gold Reagents, should not be vortexed since this could lead to conformational changes which affect the activity.
FAQ ID -2844