For the isolation of total RNA from stool and gut material
Fast and easy protocol isolates high-quality total RNA in less than 45 minutes
Efficient isolation of total RNA from difficult microbiome samples
Highly pure RNA isolation with Inhibitor Removal Technology
Simple co-isolation of miRNA and DNA using an on-column procedure
Get high-quality RNA fast, free of lingering PCR inhibitors with the RNeasy PowerMicrobiome Kit. Designed for purification of total RNA from stool, dried feces, gut material, contaminated buccal swabs and secretions, the RNeasy Power Microbiome Kit isolates RNA that is ready for demanding downstream applications including RT-PCR, qPCR and RNA-seq. Digested food, heme from lysed red blood cells in stool and other PCR inhibitors are no match for the Inhibitor Removal Technology that is included in the kit. The RNeasy PowerMicrobiome Kit also isolates miRNA and can optionally co-isolate DNA using a simple, on-column procedure using reagents provided with the kit.
Isolation of RNA using the RNeasy PowerMicrobiome Kit can be automated on the QIAcube Connect.
RNeasy PowerMicrobiome Kit was formerly sold by MO BIO as PowerMicrobiome RNA Isolation Kit.
Figure 1. High-quality, intact total RNA isolated from human stool using the RNeasy PowerMicrobiome Kit.
Healthy volunteer samples were collected and total RNA purified using the RNeasy PowerMicrobiome Kit. Profiling and quantification of 1 µl of RNA was performed using an Agilent Bioanalyzer RNA Nano Kit chip. In the electropherogram, clear 16S and 23S bands are shown for prokaryotic rRNA. RNA processed through the RNeasy PowerMicrobiome Kit was demonstrated to be high-quality by an RIN of 9.4. (Data kindly provided by C. Vincent, McGill University, Montreal, Canada.)
Figure 2. RNeasy PowerMicrobiome Kit isolated high-quality total RNA from canine stool samples.
Experimental RNA preservation solution was used to preserve fresh stool samples at room temperature or on ice. Collection and processing occurred within the same 24-hour period. RNeasy PowerMicrobiome Kit was used to isolate total RNA. Each sample (10 µl per) was examined on a 1% agarose gel.