FAQ-400
Has RNAi been successful using siRNA in Zebrafish and Xenopus?
Here are examples of references that describe the inhibition of gene expression by siRNA in Xenopus and Zebrafish:
- Wargelius A, Ellingsen S, Fjose A. Double-stranded RNA induces specific developmental defects in zebrafish embryos. Biochem Biophys Res Commun. 1999 Sep 16; 263(1): 156-61
- Zhou Y, Ching YP, Kok KH, Kung HF, Jin DY. Post-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA. Nucleic Acids Res. 2002 Apr 1; 30(7): 1664-9
- Zhao, Y Cao, M Li, and A Meng Double-stranded RNA injection produces nonspecific defects in zebrafish. Dev Biol, Jan 2001; 229(1): 215-23."
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FlexiTube siRNA Premix
FlexiTube siRNA Premix is an optimized mixture of siRNA and transfection reagent. Premixing of siRNA and reagent saves time by eliminating mixing and complex formation protocol steps. In addition, use of FlexiTube siRNA Premix removes the effort involved in estimating optimal siRNA-reagent ratios. FlexiTube siRNA Premix is available for human and mouse genes.
FlexiTube siRNA
Two different FlexiTube products are available: FlexiTube siRNA and FlexiTube GeneSolution. FlexiTube siRNA is a cost-effective solution for RNAi analysis of small numbers of genes. siRNAs are provided in 5 nmol or 20 nmol amounts for human, mouse, or rat genes, or in economical 1 nmol amounts for human and mouse genes. FlexiTube GeneSolution is a gene-specific package of 4 preselected siRNAs (1 nmol) for a target gene. FlexiTube GeneSolutions enable researchers to use multiple siRNAs for each target ensuring reliable results. FlexiTube siRNA and FlexiTube GeneSolutions are designed using innovative HP OnGuard siRNA Design and are available at QIAGEN's GeneGlobe Web portal.
FlexiPlate siRNA
FlexiPlate siRNA provides highly flexible RNAi screening and is available at 0.1 nmol, 0.25 nmol, and 1 nmol scales in 96-well plates, and at 0.1 nmol and 0.25 nmol scales in 384-well plates for a choice of target genes. For maximum flexibility, siRNAs can be selected and plate layout specified at the GeneGlobe Web portal. Lists of preselected siRNAs are also available for many gene families. siRNAs have been designed using HP OnGuard siRNA Design, which incorporates neural network technology, proprietary homology analysis, and advanced features, such as 3' UTR/seed region analysis, asymmetry, SNP avoidance, and interferon motif avoidance.Note: QIAGEN does not provide FlexiPlate siRNA in pools.
HP Custom siRNA
HP Custom siRNA is an siRNA synthesis option that provides for specific siRNA requirements, including siRNA for multiple species, specific splice variants, and non-human, -mouse, and -rat genes. HP Custom siRNA provides highly pure siRNA in 20 nmol amounts. Available fluorescent labels include Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 647, Cyanine 570, Cyanine 670, fluorescein, and rhodamine dyes. Modification options include amino linkers, thio linkers, and biotin, dabcyl, and phosphate modifications.