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QIAseq Fusion XP Targeted Panels

Comprehensive oncology profiling combining gene fusion detection, gene expression analysis and single nucleotide variant (SNV) calling in just one assay

Features

  • Incorporates QIAseq single primer extension (SPE) chemistry for design flexibility
  • Unique molecular indices (UMIs) remove PCR duplicates and maximize sensitivity and specificity
  • Fully customizable
  • Streamlined workflow and single-tube enrichment pool for easy setup
  • Excellent solution for difficult sample types such as FFPE

Products

QIAseq Fusion XP Targeted Panels are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
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QIAseq RNA Fusion XP Panel (12)

Cat. No. / ID: 334602

Kit containing ALL reagents (except indices) sufficient to process 12 samples for RNA sequencing analysis of gene fusions, gene expression and RNA SNP/InDel
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QIAseq RNA Fusion XP Panel (96)

Cat. No. / ID: 334605

Kit containing ALL reagents (except indices) sufficient to process 96 samples for RNA sequencing analysis of gene fusions, gene expression and RNA SNP/InDel
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QIAseq RNA Fusion XP Extended Panel (96)

Cat. No. / ID: 334645

Kit containing ALL reagents (except indices) sufficient to process 96 samples for RNA sequencing analysis of gene fusions, gene expression and RNA SNP/InDel
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QIAseq 8-Unique Dual Index Set A (48)

Cat. No. / ID: 333715

Box containing unique dual-indexed adapters, for indexing up to 8 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 48 samples; 1st of 2 sets required for multiplexing up to 16 samples
DKK 11,460.00
Add to cart
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QIAseq 8-Unique Dual Index Set B (48)

Cat. No. / ID: 333716

Box containing unique dual-indexed adapters, for indexing up to 8 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 48 samples; 2nd of 2 sets required for multiplexing up to 16 samples
DKK 11,460.00
Add to cart
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QIAseq 96-Unique Dual Index Set A (384)

Cat. No. / ID: 333725

Box containing unique dual-indexed adapters, for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 384 samples; 1st of 2 sets required for multiplexing up to 192 samples
DKK 49,390.00
Add to cart
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QIAseq 96-Unique Dual Index Set B (384)

Cat. No. / ID: 333735

Box containing unique dual-indexed adapters, for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 384 samples; 2nd of 2 sets required for multiplexing up to 192 samples
DKK 49,390.00
Add to cart
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QIAseq 12-Index L (48)

Cat. No. / ID: 333764

Box containing oligos for indexing up to 12 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 48 samples
DKK 9,345.00
Add to cart
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QIAseq 96-Index L (384)

Cat. No. / ID: 333777

Box containing oligos for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 384 samples
DKK 41,230.00
Add to cart

Product Details

QIAseq RNA Fusion XP enables the combined analysis of RNA fusion with SNV and gene expression from a single sample, allowing you to gain the complex RNA-seq insights your research demands. QIAGEN has leveraged our expertly curated design content and unparalleled custom assay capabilities to provide unprecedented NGS flexibility. Many other fusion detection systems suffer from a high false-positive (FP) rate, resulting from the high sensitivity these analyses demand. However, QIAseq Fusion XP Targeted Panels stand out among the rest and provide both an extremely low FP rate and high fusion detection sensitivity.

Incorporating our SPE chemistry, which requires just one gene-specific primer per target, QIAseq Fusion XP Targeted panels offer impressive flexibility for targeting fusions and for dense overlap of SNV regions of interest. Plus, all kits are optimized for use with our unique dual index (UDIs) adapters for use with Illumina® sequencing systems. Our collection of catalogued panels includes focused targets for sarcoma, leukemia, lymphoma and other oncology applications, and our custom panels are fully customizable and support both gene-level and transcript-based designs.

Performance

The QIAseq RNA Fusion XP Panels deliver outstanding efficiency and sensitivity. An analysis using only 5 ng of control RNA as the input resulted in the successful detection of eighteen clinically relevant RNA gene fusions (see figure “ Outstanding efficiency and sensitivity”).

Relative gene expression quantitation by NGS is highly influenced by the read count, and even with the use of unique molecular indices (UMIs), it will still be affected by the amount of input RNA, the sequencing read depth and other factors. But the QIAseq Fusion XP Targeted Panels enable robust gene expression analysis. The built-in internal reference control make it possible to normalize the expression levels for various amounts of sample input (see figure “ High correlation gene expression over a 5-fold difference in RNA input”).

The QIAseq RNA Fusion XP Panels also provide outstanding sensitivity for detection of SNP/InDels. When used to analyze GM12878 RNA samples, the panels achieved 93.4% sensitivity for SNPs (VMF >=5%) within the covered and expressed target region. Furthermore, since cancer-specific markers often reside within exosomes, the panels can also detect SNPs in exoRNA (see figure “ High sensitivity SNP/InDel detection”).

See figures

Procedure

Isolated total RNA (10 ng or even less), is reverse transcribed with high efficiency into first-strand cDNA, followed by a robust second-strand cDNA synthesis. In the subsequent library construction step, adaptor complexes containing unique molecular indices (UMIs) and sample indices are incorporated into the dsDNA generated in the previous step. Next, QIAseq’s SPE chemistry is used to enrich the targets of interest, which can be any combination of gene fusions, gene expression and SNP/InDel targets. Finally, the libraries are amplified via fast universal PCR, in which a second index is added (dual indexing).

Applications

QIAseq RNA Fusion XP Panels use single primer extension (SPE) and unique molecular index (UMI) technologies in NGS to help identify and characterize fusion gene events, gene expression, and SNP/InDel at the RNA level.

Supporting data and figures

Resources

Brochures & Guides (2)
State-of-the-art technologies to fast-track and streamline NGS workflows

Next-generation sequencing using QIAGEN’s QIAseq – a powerful tool for human identification


Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Kit Handbooks (1)