Ligationsrechner

Dieses Tool bestimmt die erforderliche Insert-Masse für verschiedene molare Insert:Vektor-Verhältnisse innerhalb des für typische Ligationsreaktionen geeigneten Bereichs.

icon_0330_cc_gen_calculator-sZielsequenz berechnenGeben Sie die Daten der interessierenden Zielsequenzen ein, um Sequenzierungsberechnungen zu erhalten
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Erforderliche Insert-DNA-Masse [g] = Vektor-DNA-Masse [g] x {Insert-DNA-Länge / Vektor-DNA-Länge} x gewünschtes molares Verhältnis Insert zu Vektor

Hinweise: Das gewünschte Verhältnis ist eines aus dem Set {1, 2, 3, 5 ,7}

Die Insertlänge sollte geringer sein als die Vektorlänge

Mehr erfahren

Zunächst werden Insert- und Vektor-DNA mit Restriktionsendonukleasen verdaut, um DNA-Fragmente mit klebrigen (oder stumpfen) Enden zu erzeugen. Anschließend werden die gewünschten DNA-Fragmente isoliert und danach durch Behandlung mit DNA-Ligase zusammengefügt. Zum Schluss wird der resultierende intakte Vektor in kompetente E. coli-Zellen transformiert, und die Transformanten werden anhand geeigneter genetischer Auswahlmethoden identifiziert. 

 

Möchten Sie mehr über den Schritt-für-Schritt-Prozess der DNA-Ligation und ihre typische Reaktion erfahren? Erfahren Sie mehr.

Entdecken Sie Enzyme, die Sie für die Ligation von DNAs verwenden können.

 

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Entdecken Sie qualitativ hochwertige Produkte für verschiedene molekularbiologische Applikationen.

Empfohlene Produkte

AllTaq PCR Core Kit (250 U)
CAT NO./ID 203123
AllTaq PCR Core Kit (250 U)
50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water
194,00 €
Taq DNA Polymerase (250 U)
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Taq DNA Polymerase (250 U)
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End-Repair Mix (low concentration)
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AdnaMag-L
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Pathogen Lysis Tubes L
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Cooling Adapter, LC capillaries 32, Qsym
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Adapter for holding up to 32 capillaries (20 µl) for the LightCycler; for use with the QIAsymphony AS (software version 3.1 or higher)
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artus VZV LC PCR Kit (96) CE
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artus VZV LC PCR Kit (96) CE
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artus EBV LC PCR Kit (24) CE
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artus EBV LC PCR Kit (24) CE
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AdnaMag-L
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QIAseq Targeted DNA HC Panel (12)
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QIAseq Targeted DNA Pro HC (12)
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QIAseq Targeted RNAscan HC Panel (12)
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T7 DNA Ligase (900,000 U)
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Taq-B DNA Polymerase (10,000 U)
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TaqNova Polymerase UCP (1000 U)
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RNase A (17,500 U)
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2.5 ml (100 mg/ml; 7000 units/ml, solution) Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.
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HotStarTaq DNA Polymerase (250 U)
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QIAGEN Protease (7.5 AU)
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