Digital PCR

QIAcuity dPCR products

Making acuity our focus 

Making digital PCR both scalable and practical, and reducing the time to scientific insight by integrating the qPCR workflow into a superior all-in-one walkaway solution, is one of the core ideas driving the development of the QIAcuity.

Video recorded prior to COVID-19

Product Groups – Discover featured QIAcuity products

Expert Custom Assay Design

Let us do all the hard work. Get expert-designed custom single or multiplex dPCR and PCR assays for mutation detection, CNV analysis, gene expression and species detection.

A woman in a lab coat in a lab looking at a tablet
Guide to digital PCR multiplexing
All you need to know about dPCR multiplexing, from benefits and applications to tips and products for adopting this approach in your lab.
  QIAcuity One QIAcuity Four  QIAcuity Eight
Plates processed 1 4  8
Detection channels (multiplexing) 2 or 5 5  5
Thermocycler(s) 1 1  2
Time to result Approx. 2h First plate approx. 2 h
Every ~80 min a following plate
 First plate approx. 2 h
Every ~40 min a following plate
Throughput (samples processed in a work day) Up to 384 (96-well)
Up to 96 (24-well) 
Up to 672 (96-well)
Up to 168 (24-well) 
Up to 1248 (96-well) 
Up to 312 (24-well) 
Don’t like going through 234987 product search results?
Then you will love the QIAcuity ecosystem. Explore our portfolio of nanoplates, master mixes, assays and kits for digital PCR all on ONE page that is actually fun to navigate.

Unveiling the magic inside

Reimagining familiarity, ease of use and finer resolution, we have developed these nanoplates with superior partitioning, meaning unparalleled precision and sensitivity gain for your assays.

  Specifications Applications
Nanoplate 26K 8-well 8-well x approx. 26,000 partitions Rare mutation detection, liquid biopsy, pathogen detection, etc.
Nanoplate 26K 24-well 24-well × approx. 26,000 partitions
Rare mutation detection, liquid biopsy, pathogen detection, etc.
Nanoplate 8.5K 24-well 24-well × approx. 8500 partitions
CNV determination, NGS library quantification, genome edit detection, etc.
Nanoplate 8.5K 96-well 96-well × approx. 8500 partitions
CNV determination, NGS library quantification, genome edit detection, etc.
  Specifications Applications
QIAcuity Probe PCR Kit For single and multiplex use (1- to 5-plex) Optimized for best performance in nanoplate microfluidic
Includes special reference dye needed for dPCR analysis and counting analyzable partitions
QIAcuity EG PCR Kit EG-based master mix Optimized for best performance in nanoplate microfluidic
Includes special reference dye needed for dPCR analysis and counting analyzable partitions
QIAcuity UCP Probe PCR Kit Nucleic acid-depleted, ultra-clean reagents Optimized for processes that are prone to contaminating background DNA
Includes ready-to-use Master Mix for microfluidic use in singleplex or up to 5-plex analysis
QIAcuity OneStep Advanced EG Kit For dye-based one-step RT-PCR with EvaGreen With concentrated PCR Master Mix for loading more sample
Includes HotStart RT Enzyme for room-temperature reaction setup and simultaneous Nanoplate setup
QIAcuity mericon Food Testing Kits For digital PCR authentication of food, animal feed
and certain pharmaceutical product components
Suitable for detection of sheep, chicken, cattle and pig DNA
Includes internal control for identifying potential inhibition
QIAcuity OneStep Avanced Probe Kit For one-step RT-PCR Optimized for quantification of RNA or RNA+DNA targets
Includes HotStart Reverse
Transcription (RT) Enzyme for room-temperature reaction setup and simultaneous Nanoplate setup
QIAcuity Mycoplasma Quant Kit and Standards For probe-based detection of 110 mycoplasma species Enables a Pharmacopeia-compliant workflow Includes 10 different
Mycoplasma CFU Standards and an internal control as overall control of the reaction

*For RNA samples and cDNA synthesis, the QuantiTect Reverse Transcription Kit is recommended.

Not sure which digital PCR products to pick for your application?
This smart online tool can help you find the right instrument and consumables to match your workflow needs.

Publications

Browse a growing list of articles featuring QIAcuity dPCR products.

How to choose a QIAcuity Digital PCR System

Together with its nanoplates, kits and assays, each instrument delivers high performance and data quality, with flexible throughput and multiplexing. Ask the right questions to evaluate your needs.

  • What applications will you perform most frequently?
  • Which instrumentation options best enable your scale of study?
  • What are the costs for product care beyond the initial investment?

For further guidance about comprehensive product care when making your decision, download the QIAcuity Service Brochure.

Can your research benefit from having QIAcuity?

Make the right choice for your laboratory.

*The GT-Digital SARS-CoV-2 Wastewater Surveillance Assay For QIAcuity is currently available in the United  States and Canada. Contact your country representative for product availability details. GT Molecular is The Molecular App Company™.

The QIAcuity is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Therefore, the performance characteristics of the product for clinical use (i.e., diagnostic, prognostic, therapeutic or blood banking) is unknown.