QIAamp MinElute Virus Vacuum Kit

从血浆,血清或无细胞体液中同时纯化病毒DNA和RNA

  • 快速纯化高品质病毒DNA和RNA
  • 无需有机提取或乙醇沉淀
  • 重复性好,产量高
  • 完全去除污染物和抑制剂

QIAamp MinElute Virus Vacuum Kit使用快速真空操作简化纯化病毒DNA和RNA的操作流程。QIAamp MinElute Virus Vacuum Kit起始样品体积可多至0.5 ml。该试剂盒同时具备硅胶膜选择性结合DNA的特性,以及20–150 µl的灵活洗脱体积。

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QIAamp MinElute Virus Vacuum Kit (50)
For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)
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The QIAamp MinElute Virus Vacuum Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp MinElute Virus Vacuum procedure.|High sensitivity in PCR.|High sensitivity in RT-PCR.|
|The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. For the spin procedure, 95% probit values for 40 samples were 27.25 IU/ml and for the vacuum procedure, they were 9.30 IU/ml. |The percentage of positive samples at low virus titers is shown. Nucleic acids from an RNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subject to RT-PCR. For the spin procedure, 95% probit values for 32 samples were 22.84 IU/ml and for the vacuum procedure, they were 9.46 IU/ml.|
Performance

纯化的病毒核酸不含蛋白质、核酸酶或其他杂质,适用于PCR和RT-PCR等多种敏感的下游应用(参见"High sensitivity in PCR"和"High sensitivity in RT-PCR")。

使用QIAamp MinElute Virus Vacuum Kit纯化的病毒核酸适用于多种下游应用,包括:

PCR和real-time PCR
传染性疾病研究
Principle

QIAamp MinElute Virus Vacuum Kit使用快速离心操作,简化了从血浆、血清或无细胞体液中分离病毒RNA和DNA的操作流程,无需苯酚-氯仿抽提。核酸特异性结合到QIAamp MinElute硅胶膜上,污染物流出。可通过两步高效洗涤完全去除二价阳离子或蛋白质等PCR抑制剂,再用水或试剂盒中的缓冲液洗脱结合在离心柱上的纯核酸。QIAamp MinElute技术从血浆、血清或无细胞体液中纯化的病毒RNA和DNA,可即时用于PCR和印迹实验中。

QIAamp MinElute Virus Vacumm Kit同时具备硅胶膜选择结合DNA的特性,以及20–150 µl的灵活洗脱体积。QIAamp样本制备技术完全得到认可验证。

Procedure
试剂盒提供优化的缓冲液高效效裂解样本,稳定核酸并促进DNA选择性结合到QIAamp MinElute硅胶膜上(参见"Procedure")。裂解物中添加乙醇并上样到QIAamp MinElute离心柱上。洗涤缓冲液去除杂质后,用Buffer AVE洗脱的病毒核酸可即时用于扩增反应或储存于–20ºC。经纯化的核酸不含蛋白质、核酸酶或其他污染物。
Applications

QIAamp MinElute Virus Vacuum Kit使用成熟技术可同时纯化病毒RNA和DNA。对新鲜或冷冻的血浆、血清或其他无细胞体液(500 µl)的处理在1小时内即可完成。

Feature
Specifications
Applications PCR, real-time PCR
Elution volume 20–150 µl
Format MinElute columns
Main sample type Serum, plasma
Processing Manual (vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 500 µl
Technology Silica technology
Time per run or per prep <1 hour
Yield Varies

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FAQs
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Kit Handbooks
1
For simultaneous purification of viral RNA and DNA from plasma, serum, and cell-free body fluids
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User-Developed Protocols
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This procedure has been adapted by customers and is for purification of viral RNA and DNA from plasma, serum, and cell-free body fluids using the QIAamp MinElute Virus Vacuum Kit.
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References
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