The AllTaq Master Mix Kit provides a convenient, ready-to-use 4x concentrated master mix formulation, which allows for a high template input volume to further boost sensitivity. The AllTaq PCR Core Kit offers AllTaq DNA Polymerase, PCR buffer, dNTPs, MgCl2
and Q-solution in separate tubes, if optimization of the PCR protocol should be desired.
The orange Master Mix Tracer is optionally added to master mix or PCR buffer. When template dyed with the blue Template Tracer is added to the master mix, the color of the solution changes from orange to green, providing a visual indication of correct pipetting and reaction setup (see figure PCR procedure
). The tracer dyes allow direct loading on agarose gels after PCR and efficient tracking of the subsequent electrophoresis. The dyes run at approximately 50 bp (orange) and 4000 bp (blue) on a 1% agarose gel (see figure Integrated tracking dyes
The versatile buffer system allows ultrafast PCR, in approximately 45 minutes, using the same protocol for any standard PCR and duplex PCR. A cycling protocol is provided for long templates, between 1–9 kb.
The guard-protected, hot-start mechanism enables complete reaction setup at room temperature, eliminating the need for ice and allowing automated pipetting. After reaction setup, samples can be left at room temperature for more than 3 days before PCR cycling. Furthermore, the master mix is extremely stable, avoiding degradation during use and allowing storage at 2–8°C for up to 6 months.
The AllTaq PCR Core Kit contains Q-Solution that can be added optionally. Q-solution facilitates amplification of difficult targets by modifying the melting behavior of nucleic acids (e.g., templates with a high degree of secondary structure or are GC rich).
To further streamline PCR workflows, we recommend the QIAgility instrument for automated reaction setup and PCR analysis on the QIAxcel Advanced system.