EZ2 Connect

For end-to-end automation of nucleic acid extraction, from reagent setup to elution

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EZ2 Connect

Cat. No. / ID:  9003210

Benchtop instrument for automated isolation of nucleic acids from up to 24 samples in parallel, using sealed prefilled cartridges; includes 1-year warranty on parts and labor
The EZ2 Connect is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Virtual Demo
Explore the virtual tour to learn more and see this product in action.

Features

  • Purify DNA and RNA from as many as 24 samples at the same time
  • Boost reproducibility and convenience with prefilled reagent cartridges
  • Cut down pretreatment efforts with on-board heating and reagent pipetting
  • Achieve high sensitivity with large-volume (10 mL) processing
  • Stay productive even outside the lab with QIAsphere connectivity

Product Details

The EZ2 Connect automates its prefilled cartridge-based workflows to process up to 24 samples at the same time. This benchtop instrument automatically pierces the sealed cartridges, heats samples for incubation, and automates pipetting of beads, buffers, even enzymes, to automate many pretreatment steps that were previously not automatable. Its intuitive touchscreen interface makes it easy to use, even for new lab workers. And with its remote connectivity feature, you can monitor your runs and manage your instrument even from outside the lab.

No matter what sample type you need to work with – whether FFPE samples, liquid biopsy samples, trace samples, cells, tissue, whole blood or other sample types – you can get an EZ2 Connect Kit that is run with preprogrammed protocols. Meet your research lab’s need for throughput, reproducibility and flexibility with EZ2 Connect.

Do you work specifically with forensic applications? Check out the forensics-designed features of EZ2 Connect Fx.

Performance

The EZ2 Connect helps research labs attain a whole new level of flexibility and convenience by fully automating nucleic acid extraction from a wide variety of sample types.

Boost the speed and reproducibility of your experiments by drastically reducing manual pipetting steps and replacing them with EZ2 Connect’s prefilled-reagent-cartridge system. Increase your lab throughput by processing up to 24 samples all at the same time.

The EZ2 Connect is also QIAsphere-ready, so you can monitor your runs and manage your instrument remotely, by installing the QIAsphere app on your preferred tablet or smartphone or by accessing the QIAsphere web app through your laptop or desktop browser.

Principle

The EZ2 Connect uses a piercing mechanism to open prefilled and sealed reagent cartridges. It then aspirates and dispenses samples and reagents with a 24-channel pipettor head. If heating is required during the workflow (e.g., during lysis), an on-board heating system controls the temperature of the liquids. Nucleic acid binding, washing, and elution all done automatically with in-tip magnetic bead technology (see  Expanded automation capabilities).

See figures

Procedure

Following the illustrated instructions on the touchscreen display, the user loads labware and samples onto the EZ2 Connect worktable, chooses a preinstalled protocol, and starts a run. During the run, the instrument lyses, washes, binds and elutes the nucleic acids autonomously. Once the run is over, the QIAsphere App notifies the user that the run is completed, and the user may return to the machine to collect their purified nucleic acids (see  Three simple steps to isolate DNA and RNA).

See figures

Applications

Nucleic acids purified using the EZ2 Connect and the appropriate QIAGEN kits can be used for sensitive analytical detection in downstream assays such as PCR, dPCR or NGS (see  One instrument, numerous applications).

The EZ2 Connect is compatible with EZ1&2 Kits and EZ2 Kits.

See figures

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsAutomated nucleic acid purification
FeaturesMagnetic-bead separation, on-board pipetting, heating function, automated reagent-cartridge piercing, UV-LED decontamination, remote connectivity, touchscreen display, run report management, software-guided maintenance
TechnologyMagnetic-particle technology
Main sample typeCells, tissue, FFPE, liquid biopsy samples, trace samples, whole blood
Throughput1–24 samples
Instrument dimensions57.5 cm (22.6 in) x 72 cm (28.35 in) x 56 cm (22 in) (H x W x D)

Service Plans

EZ2 Connect, PM Service

Cat. No. / ID:  9245573

One on-site preventive maintenance or inspection service visit performed by a QIAGEN service specialist, including travel, labor and parts.
EZ2 Connect, Preventive Subscription

Cat. No. / ID:  9245568

One on-site preventive maintenance (PM), including travel, labor and PM parts. Includes a 10% discount on repair services during the Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.
EZ2 Connect, Premium Agreement

Cat. No. / ID:  9245571

On-site EZ2 Connect instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one preventive maintenance or inspection service during the Premium Agreement period.
EZ2 Connect, Full Agreement

Cat. No. / ID:  9245570

On-site EZ2 Connect instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.
EZ2 Connect, Core Agreement

Cat. No. / ID:  9245569

One on-site EZ2 Connect instrument repair service and one Preventive Maintenance or Inspection Service, including travel, labor and parts, for a period of one year. Response time of five business days. Includes a 10% discount on additional repair visits during the Core Agreement period.

Resources

Technical Information and Important Notes (3)

Learn how EZ2 system supports your 21 CFR Part 11 compliance

Instructions for cleaning and disinfection of EZ2 Instruments

Previous Software Versions (2)
Version 8 (old version, compatible with software version 1.1)

Note: Unzip the folder prior to installation on the EZ2 Connect instrument, and install the *.ez2u file only.

For more information on the installation process, please refer to Section 5.3.6. (Installing New Protocols), of the EZ2 Connect and EZ2 Connect Fx User Manual.
EZ2 Connect Software
SOFTWARE (123MB)
Version 1.1.2

This is the language package corresponding to the EZ2 Connect software. For more information on the installation process, please refer to Section 5.3.7 (Language Settings) of the EZ2 Connect and EZ2 Connect Fx User Manual.


Scientific Posters (2)
Eva Haenssler, Simon Hertlein, Christina Fischer, Nicole Pickave, Martin Schlumpberger
QIAGEN Strasse 1, 40724 Hilden, Germany
Simon Hertlein, Eva Haenssler, Stefan Hey, Michael Otte, Siegfried Hauch and Martin Schlumpberger
QIAGEN Strasse 1, 40724 Hilden, Germany
Operating Software (2)
EZ2 Connect Software
SOFTWARE (248MB)
Version 1.2.0

For more information on the installation process, please refer to Section 5.3.8 (Updating software) of the EZ2 Connect and EZ2 Connect Fx User Manual.


EZ2 Off The Shelf Software (OSS) License for EZ2 Connect, EZ2 Connect Fx, and EZ2 Connect MDx
Brochures & Guides (2)
Step-by-step demos on how to use the EZ2 Connect
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Safety Data Sheets (1)
Certificates of Analysis (1)