Sequence - Am I dealing with the right genotype?
Insight offered by nucleic acid sequence
Relevance and impact on downstream analysis steps
Technologies for assessing nucleic acid sequence
While QIAGEN offers solutions for both Pyrosequencing and NGS, Pyrosequencing offers advantages over NGS for quality control and verification and validation purposes.
Pyrosequencing is a unique detection technology based on the principle of sequencing-by-synthesis and provides quantitative, real-time data without the need for gels, probes or labels. In addition to characterizing single nucleotide polymorphisms (SNPs), insertion-deletions (InDels) and unknown sequence variants, it can sensitively quantify allele frequencies and DNA methylation levels at both CpG and non-CpG (CpN) sites. Integrating detection and quantification of genetic variation into one powerful system, Pyrosequencing with PyroMark platforms outperforms other sequence-based solutions in the analysis of targeted, short DNA sequences. For a range of applications, this means reduced costs and time.
Find out more about other important parameters for sample QC: