QIAamp 96 PowerFecal QIAcube HT Kit

For automated high-throughput purification of genomic DNA from fresh or frozen stool samples that are high in PCR inhibitors


The QIAamp 96 PowerFecal QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAamp 96 PowerFecal QIAcube HT Kit (5)

Cat. No. / ID:  51531

For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, buffers
QIAcube HT Plasticware

Cat. No. / ID:  950067

Plasticware for 480 typical preps on QIAcube HT: 5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad
S-Blocks (24)

Cat. No. / ID:  19585

96-well blocks with 2.2 ml wells, 24 per case
Pathogen Lysis Tubes L

Cat. No. / ID:  19092

50 Pathogen Lysis Tubes with large beads, 1 vial of Reagent DX


  • Simple and reliable automated processing for cost and time savings
  • No hazardous organic chemicals required
  • Efficient removal of PCR inhibitors
  • High sensitivity in downstream assays

Product Details

The QIAamp 96 PowerFecal QIAcube HT Kit provides simple, automated purification of high-quality genomic DNA (human and bacterial) from up to 200 mg fresh or frozen stool samples using the QIAcube HT. The novel combination of MOBIO's patented Inhibitor Removal Technology (IRT) and proven QIAamp purification system provides an optimized DNA purification solution for microbiome, infectious disease and metabolic research applications.

The QIAamp 96 PowerFecal QIAcube HT Kit is to be discontinued shortly. Try the DNeasy 96 PowerSoil Pro QIAcube HT Kit – for improved performance. Higher yields, better purity, more accurate representation of the microbial diversity.


QIAcube HT and dedicated QIAcube HT purification kits let users increase sample purification throughput without having to compromise quality or reliability.
Effective removal of inhibitors
Stool samples are rich in PCR inhibitors such as complex polysaccharides, bile salts, lipids and urate. In the best cases, these inhibitors make it difficult to amplify targets by PCR, while in the worst cases, their presence can entirely suppress PCR signals. Therefore, effective removal of these inhibitors is critical to successful analysis of nucleic acids purified from stool samples.


The QIAamp 96 PowerFecal QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.


The QIAamp 96 PowerFecal QIAcube HT procedure is fast and simple with the QIAcube HT instrument. PCR inhibitors are removed by the MOBIO proprietary Inhibitor Removal Technology (IRT) with two precipitation steps. A combination of mechanical disruption and proteinase K digestion ensure complete lysis of even difficult-to-lyse organisms, resulting in high concentrations of unbiased DNA.
Buffering conditions are adjusted to provide optimal DNA binding, and the lysates are loaded onto the QIAamp 96 plate. As vacuum is applied, DNA binds to the QIAamp membrane, while contaminants pass through. Remaining impurities are removed in four efficient wash steps. Pure DNA is eluted under vacuum in a single step in 100 µl of Buffer ATE. No hazardous organic chemicals are used, increasing laboratory and personal safety.


The high-quality nucleic acids are ready to be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease and metabolic research applications.


Time per run96 samples in approximately 120 minutes 24 samples in approximately 60 minutes
Sample amountUp to 200 mg fresh or frozen stool
Throughput24-96 samples in increments of 8 samples
Elution volume100 μl


Operating Software (1)
Files necessary for configuring the QIAcube HT Prep Manager Software to process QIAamp 96 PowerFecal QIAcube HT Kits. Not compatible with QIAcube HT Operating Software 4.17.

For kit configuration installation instructions, please refer to the QIAcube HT User Manual  chapter 4.4.5.
Kit Handbooks (1)
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
Safety Data Sheets (1)
Quick-Start Protocols (1)
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
Brochures & Guides (2)
Certificates of Analysis (1)


Can I run the QIAcube HT with the QIAxtractor software or vice versa?

No, the two software applications are not interchangeable.


Additionally, the QIAcube HT and QIAxtractor software cannot be installed on the same computer. The QIAcube HT software versions is v4.17 or higher, while the QIAxtractor software version is v4.16 or lower.

FAQ ID -9102
For what batch size is the QIAcube HT Plasticware designed?
The QIAcube HT Plasticware content is calculated for use with the extraction protocol working in batches of 96 samples. If running smaller batches and/ or the transfer protocol, additional consumables are required. 
FAQ ID - 4010
Can I upgrade a Corbett CAS-1820 to a QIAcube HT?

No, this upgrade is not possible.

FAQ ID -9104
What file is required for reporting a QIAcube HT or QIAxtractor issue?

The Support Packages are important when reporting an issue to QIAGEN Technical Services. Upon completion of each run, the software automatically saves a support package.


To retrieve a support package, click Help in the QIAcube HT or QIAxtractor software and select Choose support package. In the new windows, select the support package that corresponds to the issue and send it to QIAGEN Technical Services.


FAQ ID -9100
I am getting vacuum errors on my QIAcube HT. What should I do?

1. Check the vacuum tubing to make sure it is not pinched or obstructed in some way. Sometimes the tubing can become pinched against the bench top when the vacuum station is placed too far from the instrument.

Waste bottle tubing

2. Make sure that the channeling adapter is properly placed. Check if the carriage is moving the plate completely from the waste chamber position to the elution chamber position. If the calibration seems incorrect, the instrument may need to be re-taught at this position.


3. When using less than a full plate, make sure all the unused columns are covered with an adhesive seal.

Also, make sure each column of 8 samples is full. When processing a number of samples that results in < 8 samples per column, use water as a substitute in the empty positions until the column is filled. This ensures even vacuum across all unsealed well positions.

4. Make sure correct plates are used for the selected protocol.

5. When the vacuum is on, please listen for any hissing noise from the tubing that is coming down to the waste container. This could indicate leaks in the tubing.

If the issue persists, please contact QIAGEN Technical Service.

FAQ ID -9106
What do I do if the QIAcube HT or QIAxtractor software runs in virtual mode while the instrument is connected to the computer?

Make sure the QIAcube HT or QIAxtractor software uses the same COM port number as assigned to the QIAcube HT or QIAxtractor instrument on the computer. The COM port setting can be checked by clicking Options\Robot Setup\Select COM port in the QIAcube HT or QIAxtractor software.


Ensure that the cable between the instrument and the computer is securely inserted. Power on the instrument first, and then launch the QIAxtractor/QIAcube HT software. 


The instrument has both RS-232 (serial) and USB interfaces for computer connection. Try an RS-232 cable if the communication cannot be established between the instrument and computer with a USB cable.


If the issue persists, please contact QIAGEN Technical Services.


FAQ ID -9101