The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions?
FAQ ID -2750

It is not reliable to interpret your results when the EpiTect ChIP qPCR primers show high Ct, especially over 34, in all three fractions: input, specific antibody and control IgG. That is because

1). Error occurs with high Ct;

2) The PCR with high Ct might not produce the real amplicon. It could be just the background noise. With our ChIP PCR Primers, you should see Ct at 24-30 at least in the input fraction with recommended ChIP starting material.

In addition, our ChIP PCR Primers meet all the essential requirements for accurate and reliable delta-delta Ct calculations using real ChIP DNA samples. Also they are available within a 30 kb promoter region around every transcription start site (TSS) in the RefSeq database.