The MiSeq FGx System achieves exceptional data quality by employing a proprietary, reversible terminator‑based method that detects single bases as they are incorporated into massively parallel DNA strands. Fluorescent terminator dyes are imaged as each deoxynucleotide triphosphate (dNTP) is added and then cleaved to allow incorporation of the next base. With all four reversible, terminator-bound dNTPs present at each sequencing cycle, natural competition among bases minimizes incorporation bias.
The MiSeq FGx Reagent Micro Kit and the MiSeq FGx Reagent Kit are identical in manufacture. With both kits, the best-in-class sequencing-by-synthesis (SBS) technology remains unaltered. The only difference is the surface area of the flow cell available for imaging. The MiSeq FGx System images the micro flow cell at approximately 35% of the standard flow cell area for a total output of 5 million reads.