The QIAamp cador Pathogen Mini Kit combines the features of several sample preparation kits based on spin-column technology to permit the purification of viral RNA and DNA and bacterial DNA from animal fluid and tissue samples. The spin-column procedure uses optimized buffers and enzymes to lyse samples and stabilize the pathogen nucleic acids. This RNA and/or DNA binds to the QIAamp silica membrane, while contaminants pass through. Wash buffers are used to completely remove PCR inhibitors, such as divalent cations and proteins, and the pure pathogen nucleic acids are then eluted in Buffer AVE.
QIAamp cador Pathogen Kit technology yields pathogen RNA and DNA from animal samples that is ready for use in downstream applications such as real-time PCR and RT-PCR.
In contrast to other kits based on silica membranes, up to 200 µl of undiluted animal whole blood samples can be processed with the QIAamp cador Pathogen Mini Kit without clogging the filter. See the table "Key features of the QIAamp cador Pathogen Mini Kit".
|Format and processing ||Mini spin columns, manual centrifuge protocol, or automated centrifuge protocol using the QIAcube|
|Target nucleic acids ||Viral RNA, viral DNA, and bacterial DNA|
|Sample source types ||Animal whole blood, serum, plasma, other fluids, swabs, washes, and tissues|
|Sample size ||Up to 200 μl or 25 mg|
|Preparation time ||20 to 40 minutes|
|Elution volume ||50 to 150 μl|