This webinar covers various fundamental aspects of performing a successful digital PCR (dPCR) assay on the QIAcuity instruments. An essential parameter to consider in designing a dPCR assay is the choice of templates and their concentrations. Here, we describe the various templates used in digital PCR and focus on their quantitative and qualitative aspects. Further, an insight into a few example applications of the technology, including absolute quantification, will be provided, and underlying calculations and statistics involved in the analysis will be explained. Lastly, we will walk you through the QIAcuity Software Suite, explaining the different views and interpretations of sample data.
About the speaker
Andreas Missel, Ph.D., Director R&D
Dr. Missel is the Director of Research and Development at QIAGEN, responsible for the continuous innovation of nucleic acid modification and amplification technologies and products, such as reagents and assays for QIAGEN's standard and real-time PCR, RT-PCR, HRM solutions, and, most recently, digital PCR. Dr. Missel received his Ph.D. in molecular biology and biochemistry from the Gene Center at the University of Munich. He has worked in various capacities in the biotechnology industry for more than 20 years.
Date of recording:Thursday, July 23, 2020
Duration:60 minutes
Molecular Biology Research
Digital PCR