QIAsymphony SP/AS instruments

整合了完整工作流程的自动化体系,从样本制备到反应体系构建

S_0659_IAS_QSYM

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

QIAsymphony AS

Cat. No. / ID:   9001301

QIAsymphony assay setup module: includes 1-year warranty on parts and labor
InstrumentAccessories
QIAsymphony AS
QIAsymphony SP
QIAsymphony Cabinet
QIAsymphony Cabinet SP/AS
QIAsymphony SP核酸提取纯化分析仪应与QIAsymphony Kits配合使用, 适用于试剂盒使用手册中指定的应用。QIAsymphony AS应与可与其兼容的QIAGEN Kits配合使用,适用于试剂盒使用手册中指定的应用。如果将QIAsymphony AS与非QIAGEN的试剂盒配合使用,用户应自行确认是否适用于相关应用。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 创新、易于使用的模块化系统,带有内置型触摸屏
  • 从多种类型样本中纯化DNA、RNA和蛋白
  • 自动将洗脱液转移到QIAsymphony AS模块进行反应体系构建
  • 连续上样,通过条形码扫描实现样本追踪
  • 输入输出样本列表

产品详情

使用专用QIAsymphony Kit,QIAsymphony SP核酸提取纯化分析仪可从多种起始样本中提取DNA、RNA、以及细菌和病毒核酸。QIAsymphony AS拓展了QIAsymphony SP核酸提取纯化分析仪的功能,使其与PCR反应体系自动化构建接合,再配合Rotor-Gene Q实时荧光定量PCR分析仪和QIAGEN real-time和终点法PCR试剂盒,使PCR工作流程自动化,极大提高工作效率。

原理

QIAsymphony SP核酸提取纯化分析仪能处理1–96个样本,每批24个,样本体积至多2 ml。一系列专门的QIAsymphony Kits能提高实验室效率,只需几个试剂盒配合优化的实验方案就能囊括广泛的起始材料和应用。QIAsymphony技术整合了硅胶膜纯化的速度和效率,以及磁珠纯化的便利性。

适用于一系列起始材料的专门的QIAsymphony Kits
QIAsymphony Kit 起始材料和用量
QIAsymphony DNA Kit 可从至多1000 µl人类全血、400 µl白膜层、50 mg组织(包括FFPE组织)或1 x 107个细胞中纯化DNA,可从血液中纯化病毒核酸
QIAsymphony DNA Investigator Kit 为人类身份鉴定和法医学纯化参考和个案样本
QIAsymphony DSP Virus/Pathogen Kit 从血浆、血清和脑脊液中纯化病毒核酸,从呼吸道和泌尿生殖系统的样本中纯化病毒或细菌核酸
QIAsymphony DSP AXpH DNA Kit 运用AXpH技术从液体细胞学样本中纯化DNA
QIAsymphony RNA Kit 从50 mg组织(包括FFPE组织)或1 x 107个细胞中纯化总RNA和miRNA
QIAsymphony PAXgene Blood RNA Kit* 从在PAXgene Blood RNA Tubes中收集的2.5 ml人类全血中纯化细胞内RNA(包括miRNA)
QIAsymphony mericon Bacteria Kit 从500 μl富集培养物中纯化细菌核酸
QIAsymphony DSP DNA Kit 从至多1000 µl人类全血、400 µl白膜层、组织(包括FFPE组织)、细菌和细胞培养液中纯化DNA,从血液中纯化病毒核酸
QIAsymphony Certal Kits 从纯化缓冲液、细胞培养基上清样本和疫苗制备样本中纯化残留的宿主细胞DNA和病毒核酸
* 每次可处理多至72个样本。

将QIAsymphony SP核酸提取纯化分析仪拓展到QIAsymphony AS,实现了整个流程的自动化。此系统可消除手动移液步骤产生的人为误差,非常适合于每日需要进行复杂的PCR分析的实验室。QIAsymphony AS可保持试剂、洗脱液和分析试剂的活性,确保安全、可重复性的分析体系构建。 QIAsymphony AS能够冷却试剂、洗脱液等,能够安全的进行反应体系构建。QIAsymphony AS每轮可构建多个反应体系,支持QIAGEN PCR和RT-PCR试剂盒。可提供上门检测分析的定制服务。

QIAsymphony SP/AS仪器设计新颖,操作过程具有杰出的安全性和标准化。吸头监控将交叉污染降至最低,内置的UV灯可对工作台面高效灭菌。对样本和试剂的条形码读取可实现纯化和分析体系构建的全程样本追踪。 可提供按时间排序的电子文档结果,包括样本ID信息、位置以及样本制备和PCR试剂的货号和有效期。多水平的用户管理可记录处理样本的是何用户。电子结果文件可通过联网的打印机自动打印,也可使用USB棒下载后存档。

QIAsymphony Cabinet SP/AS(参见" QIAsymphony Cabinet SP/AS")为正确放置QIAsymphony SP/AS仪器专门设计。QIAsymphony Cabinet SP/AS由两部分组成,分别用于放置QIAsymphony SP核酸提取纯化分析仪和QIAsymphony AS。如果有QIAsymphony SP核酸提取纯化分析仪和QIAsymphony AS仪器,需要柜子的两部分并列放置。如果只有QIAsymphony SP核酸提取纯化分析仪,只需要QIAsymphony Cabinet SP。QIAsymphony Cabinet SP和QIAsymphony Cabinet AS都有废物隔层,用于盛装工作台弹出的用过的吸头。

查看图表

程序

即用型QIAsymphony SP核酸提取纯化分析仪只需最简单的设置。已装有QIAsymphony SP核酸提取纯化分析仪的实验室可再添加QIAsymphony AS模块,简单升级系统。QIAsymphony AS可与QIAsymphony SP核酸提取纯化分析仪直接接口。为了减少手工操作步骤,降低样本污染风险,在QIAsymphony SP核酸提取纯化分析仪上处理的样本可自动转移到QIAsymphony AS(完整操作模式)。为增加灵活性,QIAsymphony SP核酸提取纯化分析仪和AS也可独立操作。之前处理的样本或在不同QIAsymphony SP核酸提取纯化分析仪上处理的样本都能手动转移到QIAsymphony AS(参见" QIAsymphony workflows")。

样品制备和分析体系构建可通过触摸屏定义。简单地将需要的样本、试剂和消耗品上样到样品仓。当样品仓关闭,会进行详细的库存扫描。只能保证工作台为选择的实验方案进行正确设置。样品制备完成后,可从洗脱仓中取出纯化的核酸或蛋白,也可通过转移模块将洗脱液直接转移到QIAsymphony AS进行分析体系构建。分析体系构建后,可直接将体系转移到real-time PCR仪进行分析。

样品输入仓可兼容一系列样品形式,以适应各种工作流程和样品制备需求。可支持条形码标记的一级和二级样品管(直径10–16.9 mm)、微孔板和块板。每批可连续上样24个样本和内参,每批可选取不同实验方案,提高效率和自动化时间。QIAsymphony AS可对每次运行或每个样本设置一个或多个PCR,分离洗脱液进行检测,或对洗脱液、试剂和PCRs进行冷却。选择不同适配器可使用不同real-time PCR仪(如Rotor-Gene Q实时荧光定量PCR分析仪、96-well cyclers或32-capillary cyclers)。使用Rotor-Gene Q实时荧光定量PCR分析仪进行检测,只需在Strip Tubes或Rotor-Discs中简单设置PCR。

QIAsymphony Kits具有杰出的安全性和易用性。即用型、条形码标记的试剂筒预装有纯化流程所需的所有试剂,包括配套的酶(参见" Prefilled, sealed reagent cartridges")。快速的工作台设置可为您节约宝贵的时间。只需将两个试剂筒装入QIAsymphony仓。可使用相同或不同试剂盒的试剂筒,用于对96个样本进行不同纯化操作流程。试剂筒可被仪器自动打开,使用的试剂量适合样本数量,为您提供完整的成本控制。

查看图表

应用

QIAsymphony SP/AS适用于多种实验室应用,包括:

  • 生物医学研究
  • 人类身份鉴定
  • 兽医应用
  • 生物安全研究
  • 生命科学应用,如基因组学和蛋白质组学

软件

QIAsymphony操作软件设置了一个样本制备或分析体系构建的程序,可对使用者每一步进行指导,并可选择和采用标准化实验方案。缓冲液、试剂、预混液和对照所需体积都能自动计算,并告知使用者需要添加消耗品的数量和种类。从QIAsymphony SP向QIAsymphony AS传输样本上样信息十分方便,减少定义分析体系所需的步骤。

QIAsymphony SP/AS仪器提供标准化的连接接口。工作列表和机架文件能被输入到此仪器,在实验室收到样本之前就能设定自动化程序。分析体系构建后,样本列表可输出到real-time PCR仪。这种双向数据交换能将QIAsymphony SP/AS仪器整合到实验室信息管理系统(LIMS)中。

服务

Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?

If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.

Learn more about trade-in/trade-up opportunities.

辅助数据和图表

Specifications

FeaturesSpecifications
Power100-240 V AC, 50-60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages
Input volumeQIAsymphony SP, 100-1000 µl (application/protocol dependent)
Operating temperature15-32°C
Pollution level2
Place of operationFor indoor use only
Elution volumeQIAsymphony SP, 50–400 µl (application/protocol dependent)
Kits or Application Packs designed for this instrumentQIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits
Overvoltage categoryII
HumidityRelative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C
Instrument dimensionsQIAsymphony SP, 130 x 75 x 103 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm
ProcessingAutomated
Protocols/main application on this instrumentQIAsymphony SP — Purification of DNA, RNA, bacterial and viral nucleic acids, and 6xHis-tagged protein from a wide range of starting materials; QIAsymphony AS — PCR setup.
Storage temperature5-40°C in manufacturer's package
TechnologyQIAsymphony SP — QIAGEN magnetic-particles chemistry
Transportation temperature-25-70°C in manufacturer's package
WeightQIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg
AltitudeUp to 2000 m (6500 ft)

服务计划

QIAsymphony SP/AS, Premium Agreement

Cat. No. / ID:   9242508

On-site QIAsymphony SP/AS instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period..

QIAsymphony SP, Premium Agreement

Cat. No. / ID:   9241208

On-site QIAsymphony SP instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period.

QIAsymphony AS, Premium Agreement

Cat. No. / ID:   9244820

On-site QIAsymphony AS instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period.

QIAsymphony SP/AS, Full Agreement

Cat. No. / ID:   9242507

On-site QIAsymphony SP/AS instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.

QIAsymphony SP, Full Agreement

Cat. No. / ID:   9241207

On-site QIAsymphony SP instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.

QIAsymphony AS, Full Agreement

Cat. No. / ID:   9244819

On-site QIAsymphony AS instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.

QIAsymphony SP, Preventive Subscription

Cat. No. / ID:   9243545A

One on-site Preventive Maintenance or Inspection Service visit for the QIAsymphony SP, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

QIAsymphony SPAS Preventive Subscription

Cat. No. / ID:   9243546A

One on-site Preventive Maintenance or Inspection Service visit for the QIAsymphony SP/AS, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

QIAsymphony AS, Preventive Subscription

Cat. No. / ID:   9244817A

One on-site Preventive Maintenance or Inspection Service visit for the QIAsymphony AS, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

资源

应用与实验方案 (27)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
Supporting Software and Tools (3)
For QIAsymphony software version 5.0.4
Work List Tool
SOFTWARE (1MB)
For QIAsymphony Software version 5.0
Language Package – German
SOFTWARE (830KB)
German language package for QIAsymphony software version 5.0.4
实验方案软件 (8)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
用户使用手册 (5)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with software version 5.0
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
仪器技术参数 (10)
QIAsymphony Cabinet SP/AS documents
Differences between QIAsymphony software versions
For use with QIAsymphony SP/AS instruments (software version 5.0)
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony Cabinet SP/AS document
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Software document for QIAsymphony software version 4.0.1
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Labware Files and Documents (3)
Labware package SOW-516-0-09 for use with the QIAsymphony SP/AS instruments (software version 5.0)
For use with software version 5.0 or higher
Technical Information and Important Notes (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
Application/Protocol Documents (27)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
Protocol Files (8)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP instruments (software version 4.0 or higher)
Instrument Technical Documents (10)
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony Cabinet SP/AS documents
QIAsymphony Cabinet SP/AS document
Software document for QIAsymphony software version 4.0.1
For use with QIAsymphony SP/AS instruments (software version 5.0)
Differences between QIAsymphony software versions
Instrument User Manuals (5)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with software version 5.0

FAQ

What RNA elution volumes are available with the QIAsymphony RNA Kit?

RNA processed on the QIAsymphony SP with the QIAsymphony RNA Kit can be eluted in 200, 100, or 50 µl.

Elution in smaller volumes will provide higher concentration, but lower overall yield. Total RNA yield with elution in 50 µl will be about 20% lower compared to elution in 100 µl.

 

FAQ ID -1943
What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?

Using the Standard protocol for RNA extraction on the QIAsymphony SP, up to 400 µl of lysate can be processed while the Large-Volume protocol allows up to 800 µl of homogenate.

 

 

 

 

FAQ ID -1939
The QIAsymphony RNA kit uses RLT plus as lysis buffer. Can samples lysed in RLT and then stored in the freezer be used on the QIAsymphony?

The RLT buffer is more viscous than the RLT plus buffer. Extraction will work fine, but there may be some magnetic bead carryover to the eluate.

FAQ ID - 3533
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?

For most sample types, incubating longer than specified in the pretreatment protocol for the QIAsymphony DNA Investigator Kit does not increase yields. However, lysates can be incubated overnight without negatively affecting the quality of the extracted DNA.

 

FAQ ID -2032
Is there a charge for user training on the QIAsymphony SP System?

No. Sample handling, software, and introduction to maintenance for the QIAsymphony SP System are included in the installation training and are covered by the installation fee.

 

 

FAQ ID -1900
Are there any specific cleaning solutions recommended for the QIAsymphony SP?

The following disinfectants and detergents are recommended for cleaning the QIAsymphony SP:

  • Mikrozid® Tissues (Schülke & Mayr GmbH) — 150 tissues with 235 g agent (consists of 24g (94%) ethanol and 35g 1-propanol per 100g agent)
  • Mikrozid Liquid (Schülke & Mayr GmbH) — ethanol-based disinfectant for spraying onto surfaces (consists of 25 g ethanol and 35 g 1-propanol per 100 g agent solution)
  • RBS®-35 Detergent Concentrate (Pierce Chemical Co.) — anionic and nonionic surfactant based detergent for cleaning the touch screen and worktable door.
  • RNaseZap® (Ambion) — for complete removal of RNase contamination from glass and plastic surfaces
  • 0.1 M NaOH — for cleaning the worktable and racks to remove RNase contamination (alternative to RNaseZap)

Europe

  • Lysetol® AF (Gigsept® Instru AF) (Schülke & Mayr GmbH) — disinfectant for submerging worktable items (consists of 14 g cocospropylene-diamine-guanidine diacetate, 35 g phenoxypropanols, and 2.5 g benzalkonium chloride per 100 g Lysetol AF, with anticorrosion components, fragrance, and 15-30% nonionic surfactants

USA

  • DECON-QUAT® 100 (Veltek Associates, Inc.) — quaternary ammonium salt based disinfectant concentrate for submerging worktable items (contains 5% alkyldimethylbenzylammonium chloride and 5% alkyldimethylethylbenzylammonium chloride)

Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described above.

FAQ ID -1913
Can I program my own protocols for the QIAsymphony SP?

No. Modified QIAGEN protocols or custom protocols for QIAsymphony SP can be developed to meet your specific requirements and purchased from QIAGEN.

 

 

 

FAQ ID -1904
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?

No. The use of internal controls with the QIAsymphony Virus/Bacteria Kits is not mandatory. When using internal controls, they are added to the carrier RNA mixture (total volume of the mixture must be 120 µl).

 

FAQ ID -2040
Can uneven sample numbers be processed on the QIAsymphony SP module?

Yes. Any number of samples between 1 and 96 can be processed on the QIAsymphony SP.

 

 

FAQ ID -1906
There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do?

The barcode on the adapter at the cooling elution position is read by default. This can be changed in the configuration settings which are available from the tools menu if you have logged in as Supervisor. The option is on the tab Process SP1 and is called ”On which elution slots should the bar codes be scanned”. To disable the barcode reading, put the setting to ”none”.

FAQ ID - 3532
Can the QIAsymphony RNA Kit be used to purify RNA from blood?

For purification of RNA from blood on the QIAsymphony SP, we recommend using the QIAsymphony PAXgene RNA Kit, since it includes stabilization of gene expression profiles.

 

 

FAQ ID -1945
Why do I have to use an ACS (Assay Control Set)?
The ACS specifies the combination of a protocol and a predefined carrier RNA–internal control mixture. The user can define an internal control mixture using the QIAsymphony Management Console. A predefined internal control-carrier RNA-Buffer AVE mixture can be automatically identified by the instrument using a bar code label or it can be chosen manually.
FAQ ID -2933
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?

The QIAsymphony Virus/Bacteria Mini Kit is used with the 'Cellfree_200' and 'Complex_200' protocols.

The QIAsymphony Virus/Bacteria Midi Kit is used with the 'Cellfree_500’, 'Cellfree_1000', ‘Complex_400’ and 'Complex_800' protocols.

FAQ ID -2038
What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?

Part number for O-rings for pipetting channels of QIAsymphony SP and AS instruments is 9019168. There are 32 O-rings included in one bag.

You will also require the tool to change the O-rings. Part number for this tool is: 9019164. This tool set includes one bag of 32 O-rings.

We recommend replacing O-rings on all pipetting channels, once a month.

              

FAQ ID -3055
Can I change the language on my QIAsymphony SP system?

Yes, this is possible from software version 4.1 on. Please ask Technical Service for an update. The respective language package can be found on https://www.qiagen.com/de/resources/resourcedetail?id=7d184844-365d-43bf-95f4-0031cd5a4b8e&lang=en

FAQ ID - 3595
What sample types can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit?
Tissue and FFPE tissue can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP.
FAQ ID -2925
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?

No. All solid materials (e.g., swabs, blood card punches, chewing gum) must be removed completely from the lysate before performing automated extraction on the
QIAsymphony SP. The QIAshredder can be used to increase sample recovery from some solid materials. For more information, see the corresponding pretreatment protocol.

 

FAQ ID -2027
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?

For purification of RNA on the QIAsymphony SP, tissue samples require mechanical disruption and homogenization in the supplied lysis buffer (Buffer RLT Plus), e.g., by using the TissueLyser or TissueRuptor. Cultured cells should be lysed by vortexing in the lysis buffer contained in the kit.

 

FAQ ID -1938
How can I create a report/logfile on my QIAsymphony SP system?

Please navigate to Tools/Instrument Report/Create + Save to USB.

FAQ ID - 3594
Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?

No. We do not recommend use of carrier RNA with the QIAsymphony Virus Blood 200 Protocol.

 

FAQ ID -2163
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?

In general, magnetic particles are not carried over into QIAsymphony eluates. However, if high amounts of genomic DNA are purified (large number of white blood cells in the sample) magnetic-particle carryover may be observed. If carryover does occur, magnetic particles in eluates will not affect most downstream applications. If magnetic particles need to be removed before performing downstream applications, tubes or plates containing eluates should first be placed in a suitable magnet and the eluates transferred to a clean tube.

 

FAQ ID -2166
How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?

Tissue: If no information about the expected yield is available, we recommend starting with 25 mg sample material. Depending on the yield obtained, the sample size can be increased in subsequent preparations to a maximum of 50 mg.

FFPE: Up to 4 sections, each with a thickness of up to 10 µm, or 8 sections with a thickness of up to 5 µm and a surface area of up to 250 mm2, can be combined in one preparation.

FAQ ID -2927
How should DNA isolated on the QIAsymphony be stored?

Eluates obtained on the QIAsymphony SP can be stored at 2–8°C for 3 days before use in downstream applications and should be placed at –20°C or –80°C for long-term storage.

 

 

FAQ ID -2165
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?

Plasma, serum, and CSF samples can be processed using the Virus Cellfree protocols of the QIAsymphony Virus/Bacteria Kits. Respiratory samples (sputum, BAL, aspirates, dried swabs, transport media), and urogenital samples (urine, urogenital swabs, transport media) can be processed using the Pathogen Complex protocols.

 

FAQ ID -2036
Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using the QIAsymphony DSP DNA Mini Kit. Tissue samples are lysed by proteinase K digestion. FFPE tissue samples are deparaffinized with Deparaffinization Solution or xylene/ethanol and lysed by proteinase K digestion. Insoluble material is pelleted by centrifugation. Lysates are placed on the QIAsymphony SP for purification of DNA (see protocol sheets for details).
FAQ ID -2926
What are the expected DNA yields from tissue processed on the QIAsymphony SP?

DNA yields strongly depend on the type and source of the tissue processed on the QIAsymphony SP. Approximately 70–80 µg DNA can be isolated from 25 mg of spleen, 40–50 µg from 25 mg liver, and 10–15 µg from 50 mg muscle.

 

FAQ ID -1919
What sample input volumes can be used on the QIAsymphony SP?
200–1000 µl sample volumes can be processed on the QIAsymphony Sample Prep Module.
FAQ ID -1907
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?

The maximum amount of tissue that can be processed on the QIAsymphony SP using QIAsymphony DNA Mini Kit is 50 mg, and the maximum number of cultured cells is 1 x 107.

See details for upper limits of various starting materials in the table below:

 

Sample type Sample amount Protocol
Heart 25 mg Tissue Standard
Spleen 25 mg Tissue High Content
Lung 25 mg Tissue Standard
Liver 25 mg Tissue High Content
Kidney 25 mg Tissue Standard
Muscle 50 mg Tissue Standard
Mouse tail 0.8 cm Tissue Standard
Jurkat Cells 2 x 10e6 Tissue Standard
Jurkat Cells 1 x 10e7 Tissue High Content

FAQ ID -1917
How can I decontaminate my QIAsymphony SP / AS system?

The QIAsymphony system has an integrated UV lamp. UV decontamination should be performed daily. It helps to reduce possible pathogen contamination of the QIAsymphony SP/AS worktables. The efficiency of inactivation has to be determined for each specific organism and depends, for example, on layer thickness and sample type. QIAGEN cannot guarantee complete eradication of specific pathogens.

FAQ ID - 3714
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?

A total of 192 samples can be processed with one QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol (96 samples per reagent cartridge).

 

 

 

FAQ ID -2159
Can protocol runs on the QIAsymphony SP be interrupted and resumed?

QIAGEN protocols on the QIAsymphony SP can be stopped if there is an emergency by pressing "Pause". To confirm that you want to stop the protocol run, press "Stop". To cancel the stop protocol command, press "Continue".

Note: If a protocol run is stopped, the run cannot be restarted. Samples must be processed manually. For more information, refer to the QIAsymphony User Manual.

 

FAQ ID -1912
Is the QIAsymphony RNA Kit compatible with stabilized samples?

Yes, the QIAsymphony RNA Kit is compatible with stabilized samples. We recommend stabilizing tissue samples with RNAprotect Tissue Reagent or AllProtect Tissue Reagent, or by flash-freezing. Cultured cells can be lysed directly in cell culture dishes, or after pelleting. Alternatively, cells can be stabilized using RNAprotect Cell Reagent.


FAQ ID -1944
What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
Plasma, serum, and CSF samples can be processed using the QIAsymphony DSP Virus/Pathogen cellfree protocols. Respiratory samples (sputum, BAL, aspirates, dried swabs, transport media), and urogenital samples (urine, urogenital swabs, transport media) can be processed using the pathogen complex protocols.
FAQ ID -2928
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?

Sample volumes of 200 µl can be processed with the Virus Blood 200 Protocol using the QIAsymphony DNA Mini Kit.

 

 

FAQ ID -2156
What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP?
After pretreatment, 220 µl of the lysates must be transferred to the QIAsymphony SP to ensure that 200 µl of sample is processed.
FAQ ID -2992
What are the recommended lysis times for tissue samples on the QIAsymphony SP?
Lysis time varies depending on the tissue type processed. For recommended lysis times refer to the tissue protocol sheets. If lysis is incomplete after recommended lysis time, as indicated by the presence of insoluble material or highly viscous lysates, lysis time can be prolonged or insoluble material can be removed by centrifugation. Overnight lysis is possible and does not affect the preparation.
FAQ ID -2993
Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?

Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using QIAsymphony DNA Kits.

Samples are lysed by Proteinase K digestion in lysis buffers optimized for tissue, cultured cells, and bacteria, respectively. For lysis of gram-positive bacteria, lysozyme is used in addition to Proteinase K. Lysed samples are homogenized by pipetting up and down, and insoluble material is removed by centrifugation (see the QIAsymphony DNA Handbook for details).

 

FAQ ID -1916
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?

Samples should be processed on the QIAsymphony within 24 hours of collection. Samples should be transported and stored at 2–25°C.

 

 

 

FAQ ID -2157
Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP?

No. The specified lysate volumes are optimal for QIAsymphony DNA Investigator Kit protocols. Lower volumes (150 µL,400 µL, 900 µL, respectively) can be used.

However, to maximize yields, we recommend transferring as much of the lysate as possible.
The QIAshredder can be used to increase recovery from some sample types. For more information, see the corresponding pretreatment protocol.

 

FAQ ID -2031
What are the dimensions and weight of the QIAsymphony SP System?

The QIAsymphony SP instrument has the following weight and dimensions:

  • Weight: 178 kg (392.4 lb.); 220 kg (485 lb.) with packaging
  • Width: 130 cm (51.2 in.)
  • Depth: 75 cm (29.5 in.); 125 cm (49.2 in.) with drawer open
  • Height (door closed): 103 cm (40.6 in.)
  • Height (door open): 163 cm (64.2 in.)
FAQ ID -1901
Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?

Yes, QIAsymphony SP can be connected to a lab information management system. Result files can be exported in html format. At a later date, users will be able to import sample worklists to the QIAsymphony SP.

 

FAQ ID -1909
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?

The QIAsymphony RNA Kit enables purification of RNA from cultured cells and easy-to-lyse tissues, such as kidney, liver, and spleen on the QIAsymphony SP. A large-volume protocol is available that enables processing of larger sample amounts.

  • Cultured cells: up to 3x106 cells (standard protocol); up to 1x107 (large volume protocol)
  • Tissues: up to 20 mg of most tissues (standard protocol); up to 50 mg (large-volume protocol)

For particularly DNA-rich tissues, such as spleen or thymus, we recommend either using no more than half the maximum amount, or using DNase at a higher concentration (see the QIAsymphony RNA Handbook; additional DNase required).

FAQ ID -1937
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?

Sample volumes that can be processed with the QIAsymphony Virus/Bacteria Kits depend on the protocol:

  • 'Cellfree_200' and 'Complex_200':  200 µl
  • 'Complex_800':                            800 µl
  • 'Cellfree_1000':                           1000 µl

 

FAQ ID -2037
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?

Human whole blood samples treated with EDTA or citrate can be processed using the QIAsymphony Virus Blood 200 protocol with the QIAsymphony DNA Mini Kit. We do not recommend using heparin-treated blood samples.

 

 

 

FAQ ID -2154
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?

Yes. The DNA Tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.

 

 

 

 

FAQ ID -1921
How can I determine if a tube is suitable for clot detection on QIAsymphony?

You can find this information in the respective labware list. All tubes which require an insert in the sample input carrier or plates are not suitable.

FAQ ID - 3590
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?

Yes. QIAsymphony DNA Investigator Kit protocols purify both genomic and mitochondrial DNA.

 

 

 

FAQ ID -2030
How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?

Strongly denaturing conditions in the lysis buffer (Buffer RLT Plus) of the QIAsymphony RNA Kit keep RNases in an inactive state. Keeping homogenates on the QIAsymphony instrument at room temperature (20-25°C) for over 6 hours does not impair RNA integrity.

 

FAQ ID -1941
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?

Final elution volumes of 60 µl, 85 µl, 110 µl, or 165 µl can be selected using the QIAsymphony Virus Blood 200 protocol. Please note that the initial elution volumes are 95 µl, 120 µl, 145 µl, or 200 µl. When calculating the amount of internal control(s) as well as the titer of the processed sample, it is necessary to take into consideration the initial volume of elution buffer that is used for each sample.

 

FAQ ID -2160
Is it possible to modify QIAsymphony protocols?

No. Protocols on the QIAsymphony SP cannot be modified by users. However, protocols allow elution volumes to be selected within a predefined range. Customized protocols for QIAsymphony SP will be available on request.

 

FAQ ID -1903
Which barcodes on sample tubes are accepted by the QIAsymphony SP?

Code 39, code 128 and subtypes, Codabar

FAQ ID - 3598
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?

The High Content protocol for DNA extractions on the QIAsymphony SP is optimized for samples with an expected DNA yield of more than 30 µg. Up to 90 µg DNA can be purified from tissues with high cell densities (e.g., spleen) or large numbers of cultured cells.

 

FAQ ID -1918
Is clot detection possible on the QIAsymphony?
Yes, clot detection is possible on the QIAsymphony.
FAQ - ID 3588
How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
Samples are mixed before pipetting by the QIAsymphony SP Module.
FAQ ID -1905
Does the QIAsymphony SP system generate run reports and/or log files?

Yes. At the end of each protocol run on the QIAsymphony SP, a report file is generated that contains important information such as number of samples, liquid volumes measured during the load check, and run time. A log file is also generated that contains information about instrument actions, such as movement of the robotic arm.

 

 

FAQ ID -1911
What is the range of elution volumes on the QIAsymphony SP?

Depending on the application, elution volumes on the QIAsymphony SP range from 30 µl to 500 µl.

 

 

 

FAQ ID -1908
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?

The large-volume protocol of the QIAsymphony DNA Investigator Kit is intended for samples that can not be submerged completely during lysis in volumes less than 1 ml, as well as samples that are very absorbent.

 

FAQ ID -2034
What are the expected yields of total RNA isolated with the QIAsymphony SP?

Please see expected yields of total RNA isolated with the QIAsymphony RNA Kit on the QIAsymphony SP in the table below:

 

Sample type Sample amount Typical RNA yield (µg)*

Cultured Cells

   
HeLa, Jurkat 1 x 10e6 cells 15

Rat tissues

   
Kidney 10 mg 10-25
Liver 10 mg 20-50
Lung 10 mg 5-10
Spleen 10 mg 25-35

*RNA yields strongly depend on the type and quality of the sample material.

FAQ ID -1942
What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?

Various types of tissue, cultured cells, and bacterial pellets can be processed using the tissue protocols for the QIAsymphony DNA Kits on the QIAsymphony SP.

 

 

 

 

FAQ ID -1915
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?

Pre-treatment of samples used with the QIAsymphony Virus/Bacteria Kits can improve nucleic acid extraction for some sample types. Viscous samples (e.g., sputum, respiratory aspirates) might require liquefaction to enable pipetting. Pretreatment protocols are provided in the QIAsymphony Virus/Bacteria Handbook.

 

FAQ ID -2039
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?

Yes. Magnetic particles used in the QIAsymphony SP System will bind RNA and total DNA. If isolation of RNA-free DNA is required, RNase A must be added to the lysate before isolation of DNA.

 

FAQ ID -1920
Are QR codes recognized by QIAsymphony?
No, currently only barcodes can be used to label samples for QIAsymphony. We have no plans to introduce QR code recognition any time soon.
FAQ ID - 3625
What is the thermal output for a QIAsymphony SP and AS instrument?

Power consumption of a QIAsymphony SP instrument is 800 VA. Therefore, the approximate thermal output is 2729 BTU/h.

If the QIAsymphony SP is connected to a QIAsymphony AS, then the power consumption is 1400 VA. The thermal output is ~4777 BTU/h.

 

FAQ ID -3057
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?

QIAsymphony DNA Investigator Kit protocols are optimized for maximum sensitivity in extraction of typical forensic-casework and reference sample types, which usually have low DNA content. Due to optimized bead capacity, maximum DNA yields are approximately 3 µg.

 

FAQ ID -2028
In which tubes is clot detection possible on QIAsymphony?
Only tubes with a diameter < 15 mm can be used for clot detection on the QIAsymphony.

FAQ ID - 3589

What sample tubes can I use on my QIAsymphony SP system?

Please navigate to the Product Page of the QIAsymphony Kit you are using; for example, the QIAsymphony RNA Kit, Cat No./ID: 931636.  Refer to the resources tab and download the labware document. The labware document will list the sample tubes that have been tested for your application. Please contact Technical Service if you wish to use sample tubes not listed in the labware document.

FAQ ID - 3713
What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP?
Tissues will give increased DNA yields when processed with the high content protocol, but the low content protocol in combination with a small elution volume (50µl) may be used if high DNA concentration is required. For FFPE tissue we recommend using the low content protocol.
FAQ ID -2989
Do all QIAsymphony DNA Investigator protocols use carrier RNA?

No. Carrier RNA is only used in casework protocols for the QIAsymphony DNA Investigator Kit to enable efficient extraction of minute quantities of DNA. Carrier RNA is not used in reference protocols.

 

FAQ ID -2033
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?

No. The use of internal controls is not mandatory for use with the QIAsymphony Virus Blood 200 Protocol. If an internal control is not required, Buffer ATE without internal control is added to the samples. Buffer ATE must be placed in slot A of the sample drawer.

 

FAQ ID -2162
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
DNA yields strongly depend on the type and source of the tissue processed on the QIAsymphony SP. For FFPE tissue samples, the DNA yield depends highly on the fixation and processing of the tissue material. QIAsymphony magnetic particles copurify RNA and DNA if both are present in the sample. RNA may influence photometric analysis and may lead to overestimated nucleic acid yields. If RNA-free DNA is required, add RNase A in the step indicated in the respective pretreatment protocol (see protocol sheets for details).
FAQ ID -2990
How can the QIAsymphony SP system be decontaminated?
To decontaminate the QIAsymphony SP, refer to the QIAsymphony User Manual.
FAQ ID -1914
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?

To ensure performance with the QIAsymphony DNA Investigator Kit, lysis volumes have been optimized according to sample type. In general, large or absorbent samples require larger buffer volumes than small and non-absorbent samples.

 

FAQ ID -2035
What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?

Buffer ATE of the QIAsymphony DNA Investigator Kit is a low-EDTA elution buffer optimized for long-term storage of DNA as well as performance in sensitive downstream applications. Alternatively, casework protocols using water for elution are available.

 

Buffer ATE contains a small amount of the preservative sodium azide. If the eluate is measured for absorbance the spectrophotometer should be blanked with the buffer ATE as sodium azide absorbs strongly at 230 nm. 

 

FAQ ID -2029
Is it possible to process samples on the AS-module while SP is running?
Yes. A connected as well as an independent operation between QS-SP and QS-AS is possible.
FAQ ID -2435
How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?

Each QIAsymphony RNA Kit enables preparation of up to 192 samples (96 per reagent cartridge) using the standard protocol on the QIAsymphony SP. If only the large-volume protocol is used, the number of samples that can be prepared per kit is reduced to 96 (48 per reagent cartridge). Any combination of standard and large-volume sample preparation can be performed.

 

FAQ ID -1940
Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
Yes. The DNA tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.
FAQ ID -2991
What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?

The target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony is viral DNA from released as well as cell-associated viruses, co-purified with genomic DNA from blood cells.

 

 

FAQ ID -2158