QIAseq Targeted RNA Indexes

用于对目标RNA测序所需样本和对QIAseq Targeted RNA Panels生成的RNA文库测序进行索引

Products

QIAseq Targeted RNA Indexes 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
Image
QIAseq 96-Index L (384)

Cat. No. / ID:  333777

Box containing oligos for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 384 samples
Image
QIAseq 12-Index L (48)

Cat. No. / ID:  333764

Box containing oligos for indexing up to 12 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 48 samples
Image
QIAseq 12-Index I (48)

Cat. No. / ID:  333714

Box containing combinatorial dual-indexed adapters, for indexing up to 12 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 48 samples
Image
QIAseq 96-Index I Set A (384)

Cat. No. / ID:  333727

Box containing combinatorial dual-indexed adapters, for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 384 samples; 1st of 4 sets required for multiplexing 384 samples
Image
QIAseq 96-Index I Set B (384)

Cat. No. / ID:  333737

Box containing combinatorial dual-indexed adapters, for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 384 samples; 2nd of 4 sets required for multiplexing 384 samples
Image
QIAseq 96-Index I Set C (384)

Cat. No. / ID:  333747

Box containing combinatorial dual-indexed adapters, for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 384 samples; 3rd of 4 sets required for multiplexing 384 samples
Image
QIAseq 96-Index I Set D (384)

Cat. No. / ID:  333757

Box containing combinatorial dual-indexed adapters, for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 384 samples; 4th of 4 sets required for multiplexing 384 samples
Image
QIAseq 8-Unique Dual Index Set A (48)

Cat. No. / ID:  333715

Box containing unique dual-indexed adapters, for indexing up to 8 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 48 samples; 1st of 2 sets required for multiplexing up to 16 samples
Image
QIAseq 8-Unique Dual Index Set B (48)

Cat. No. / ID:  333716

Box containing unique dual-indexed adapters, for indexing up to 8 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 48 samples; 2nd of 2 sets required for multiplexing up to 16 samples
Image
QIAseq 96-Unique Dual Index Set A (384)

Cat. No. / ID:  333725

Box containing unique dual-indexed adapters, for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 384 samples; 1st of 2 sets required for multiplexing up to 192 samples
Image
QIAseq 96-Unique Dual Index Set B (384)

Cat. No. / ID:  333735

Box containing unique dual-indexed adapters, for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 384 samples; 2nd of 2 sets required for multiplexing up to 192 samples

特点

  1. 为Illumina和Ion Torrent测序仪提供最多96个样本的索引
  2. 每个索引可足够用于4份样本
  3. 管式包装具有灵活性,板式包装符合便利性和高通量测定的要求


产品详情

QIAseq Targeted Panels enable Sample to Insight next-generation sequencing (NGS) of DNA or RNA. These optimized solutions facilitate ultrasensitive variant and clonotype detection from cells, tissue and biofluids using integrated Unique Molecular Indices (UMIs).

In order to de-multiplex pooled libraries and assign reads to the appropriate original source library, NGS workflows require the addition of an adapter complex that includes both a common nucleotide sequence, as well as sample index oligonucleotides.

Compatible only with Illumina instruments, our Unique Dual Indexes (UDIs) are optimized for QIAseq Targeted Panel workflows, including QIAseq Targeted DNA, QIAseq RNAscan and QIAseq Immune Repertoire RNA Library Kits. Exclusively available from QIAGEN for QIAseq targeted NGS applications, QIAseq UDI Kits provide the highest level of process safety and ensure confidence in sequencing data by mitigating “index hopping” and mis-assignment of reads. The combination of both UMIs and UDIs provide QIAseq Targeted Panels with a remarkable level of accuracy. Combinatorial, non-unique dual index adapters are also available.

Need a quote for your research project or would you like to discuss your project with our specialist team? Contact Us

绩效

  • Accuracy: Innovative digital sequencing (incorporating molecular barcodes) eliminates PCR duplication and amplification artifacts to detect low-frequency variants with high confidence (see figure  Principle of molecular barcodes).
  • Specificity: The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees high specificity and accurate results.
  • Uniformity: The QIAseq Targeted DNA Panel workflow has been optimized to deliver highly uniform sequencing results, to ensure sequencing capacity is utilized very efficiently (see figure Uniformity).
  • Sensitivity: Digital DNA sequencing approach is optimized to deliver high confidence in calling low-frequency DNA variants. Over 90% sensitivity for 1% NA12878 SNP and indel on typical coding region with false positive less than 15 per mega base region when variants are detected with tiled primer design to cover complete coding region of each gene.
  • Universality: The chemistry used in the QIAseq Targeted DNA Panels and workflow is compatible with both regular and GC-rich genomic regions, allowing one to achieve 100% coverage of genes rich in GC content such as CEBPA and CCND1 (see figure  Coverage of GC-rich genomic regions).
  • Flexibility: The QIAseq Targeted DNA Panels offer a high degree of flexibility in content and sample multiplexing. Several cataloged panels have been developed for a wide range of applications. One can also build a custom panel for a specific content, or extend the contents of an existing cataloged panel. Up to 384 samples can be multiplexed using the QIAseq indices.
查看图表

原理

PCR duplicates are a major issue in targeted DNA sequencing, since, through PCR amplification, they turn unique DNA molecules into identical DNA molecules that cannot be distinguished from each other. In addition, errors from PCR amplification and sequencing process may also be present in final reads that lead to false-positive variants in sequencing results. This, in turn, results in the inability to confidently call DNA variants present at low frequencies in the starting DNA material. To overcome the issue of PCR duplicates and amplification artifacts, the QIAseq Targeted DNA Panels use digital sequencing by incorporating molecular barcodes into the starting DNA material before any amplification takes place, thereby preserving the uniqueness of the starting DNA molecules and overcoming the issues of PCR duplicates, false positives and library bias.

程序

The entire workflow of the QIAseq Targeted DNA Panels to go from extracted DNA to sequencing-ready libraries can be completed in 9 hours (see figure  Workflow). Extracted DNA is fragmented, genomic targets are molecularly barcoded and enriched, and libraries are constructed. Sequencing files can be fed into the QIAseq pipeline, a cloud-based data analysis pipeline, which will filter, map and align reads, as well as count unique molecular barcodes associated with targeted genomic regions, and call variants with a barcode-aware algorithm. This data can then be fed into IVA or QCI for interpretation.

查看图表

应用

  1. 生物标志物研究
  2. 全转录组测序数据验证
  3. 微阵列数据验证


辅助数据和图表

资源

产品介绍与指南 (2)

Next-generation sequencing using QIAGEN’s QIAseq – a powerful tool for human identification


State-of-the-art technologies to fast-track and streamline NGS workflows
应用说明 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
模板文件 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)