Preparation of the cell lysate
Clearing of bacterial lysates using QIAfilter Cartridges
DNA binding and washing on the QIAGEN-tip
Desalting and concentration by centrifugation
Desalting and concentration by QIAprecipitator Module
The eluted plasmid DNA is mixed with isopropanol and applied to the QIAprecipitator Module using the syringe provided in the kit. The module traps the precipitated DNA while the isopropanol mixture flows through. An additional ethanol wash step is recommended, to maximize DNA purity. The precipitated DNA is trapped in the QIAprecipitator as a thin layer, which allows thorough drying and removal of ethanol by simply pushing air through the QIAprecipitator with a syringe. The DNA is then eluted from the QIAprecipitator into a microcentrifuge tube with Buffer TE provided in the kit. Alternatively, any common buffer or water can be used. DNA should be stored at –20°C when eluted with water, as DNA may degrade in the absence of a buffering and a chelating agent. The DNA is ready for use in transfection, sequencing, labeling, cloning, or any other experimental procedure.