What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?

Researchers generally use small nucleolar RNAs (snoRNAs) or 5S RNA as the normalizer for miRNA.

To normalize miRNA real-time PCR data obtained with the miScript System, QIAGEN offers primer assays for 15 different snoRNAs and for 5S RNA. Any of these can be used for normalization.

 

Can’t find what you are looking for?

Browse the FAQ base with our FAQ search.