What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
The PAXgene Tissue fixation and stabilization chemistry is free of cross-linking reagents. RNA purified from PFPE is free of chemical modifications and performs similarly or identically to RNA isolated from frozen tissue. For examples of the correlation of gene expression levels in snap-frozen tissue, FFPE, and PFPE, see Figure 4 in Groelz et al., Exp Mol Pathol. 2013 Feb;94(1) and Figure 3 in Viertler et al., J Mol Diagn. 2012 Sep;14(5) .