What types of priming are compatible with EnzScript M-MLV Reverse Transcriptase RNase-H-?

EnzScript M-MLV Reverse Transcriptase RNase H is compatible with random hexamer priming, poly oligo(dT) or anchored poly oligo(dT) priming and gene-specific priming (GSP). Random hexamer priming is the most non-specific priming method and is commonly used for full coverage of fragmented RNA in library construction protocols. Poly oligo(dT) and anchored poly oligo(dT) are more specific priming methods, in which the primer hybridizes to the poly-A tail of mRNA found in eukaryotes. GSP is the most specific priming method, in which the primer hybridizes to a specific target or gene of interest. As a general precaution against RNase degradation of the RNA template, inclusion of an RNase Inhibitor (ENZ Y9240) is suggested especially when the RNA input amount is low (<100 ng).


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