If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?
The biotinylated compounds whether they be oligos or other compounds, can be removed by altering the pH in order to disrupt the Biotin/Streptavidin interaction. To do this the pH must be as high as 9.5. Once the particles have incubated in the 9.5 pH solution, simply magnetically separate the BioMag Streptavidin particles and the supernatant will contain the biotinylated oligos. When isolating double stranded DNA in this manner, be aware that this elevated pH will result in single stranded DNA product.