What is the composition of buffer QBT?
Buffer QBT is the equilibration buffer used in QIAGEN Plasmid Kits for plasmid purification and in QIAGEN Blood & Cell culture kits.
The composition of Buffer QBT is:
- 750mM NaCl • 50 mM MOPS, pH 7.0
- 15% isopropanol (v/v)
- 0.15 % Triton® X-100 (v/v)
To make 1 liter of solution, dissolve 43.83 g NaCl, 10.46 g MOPS (free acid) in 800 ml distilled water. Adjust the pH to 7.0 with NaOH. Add 150 ml pure isopropanol and 15 ml 10% Triton X-100 solution (v/v). Adjust the volume to 1 liter with distilled water. Store at 15–25°C