Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
Yes. DNA prepared using the R.E.A.L. Prep 96 Plasmid Kit is well suited for high-throughput Capillary Electrophoresis (CE) DNA sequencing, or for fluorescent BAC DNA end sequencing on ABI PRISM 377 vertical slabgel sequencers. An optimized protocol for the purification of BAC DNA suitable for end sequencing on CE machines using R.E.A.L. Prep 96 is presented in QIAGENNews article 'High-throughput sequencing of plasmid and BAC DNA prepared using the REAL Prep 96 Plasmid Kit'. Typical sequence read lengths are around 600 bp.
High sample quality is essential for most sequencing applications on CE instruments. After intensive use of such sequencers (e.g., ABI PRISM 3700, MegaBACE 1000) in QIAGEN laboratories, a large set of statistical data on the quality and performance of DNA purified using the R.E.A.L. Prep 96 Plasmid Kit is available.
If you would like to take advantage of the Sequencing Service that we offer here at QIAGEN, please contact QIAGEN Technical Service.