How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers?

VeraSeq Buffer II and VeraSeq GC buffer result in a final concentration of 1.5 mM Mg2+ in the reaction. Therefore, final Mg2+ reaction concentration may be increased according to user preference with a concentrated solution containing Mg2+.  VeraSeq 2.0 DNA Polymerase works under a broad range of Mg2+ concentrations (1.5 – 3.0 mM) but higher Mg2+ can compromise fidelity (1).     

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