Why can't I find the DNA methylation qPCR Assay for my gene of interest?

There are three possible reasons. First, there is no predicted CpG island in the promoter region (defined as 5 kb upstream to 3 kb downstream of the transcription start site) of your gene of interest. Second, part of our primer design criteria is that the amplicon should include both methylation sensitive and dependent enzyme cutting sites. If your CpG island of interest does not contain these, we are unable to design primers for it. Third, the gene symbol you input may not be correct. You will be informed of those cases in the search result page.

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